鲍曼不动杆菌对碳青霉烯类耐药性及耐药基因型分析  

Investigation on resistance to carbopenems and resistance genotypes of Acinetobacter baumannii

作　　者：赵旺胜[1] 江淑芳[2] 顾兵[1] 糜祖煌[3] 张巧娣[1] 文怡[1] 刘根焰[1] 吴海玮[4] 

机构地区：[1]南京医科大学第一附属医院检验科,江苏南京210029 [2]解放军第81医院,江苏南京210010 [3]无锡市克隆遗传技术研究所,江苏无锡214026 [4]南京医科大学现代病原生物学实验室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2006年第10期929-932,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:了解南京地区鲍曼不动杆菌耐药特点及分析碳青霉烯酶、金属β-内酰胺酶基因型。方法:K-B法测定临床分离的73株鲍曼不动杆菌对亚胺培南、美洛培南的耐药性;PCR检测碳青霉烯酶(OXA)、金属β-内酰胺酶(IMP和VIM)耐药基因,并对OXA基因进行测序。结果:73株鲍曼不动杆菌对亚胺培南、美洛培南耐药率均为10.9%;8株耐药菌中3株是IMP型,2株是VIM型,4株是OXA型,其中有1株菌含有OXA及IMP两种基因型。4例OXA型标本经测序,在Genbank中进行同源性比对,均为OXA-23亚型。结论:目前南京地区鲍曼不动杆菌的耐药性与其携带OXA-23、IMP和VIM基因相关。Objective： To survey the resistance to carbopenems and genotype of carbapenem and metallo beta-lactamase of A. baumannii. Methods：The antibiotic susceptibility to imipenem and meropenem of 73 strains of A.baumannii was tested by K-B methods. OXA,IMP and VIM resistance genes were detected by PCR, and the positive products of OXA genes were sequenced. Results： The resistance rate to imipenem and meropenem of the 73 strains of A.baumannii was both 10.9%. of the 8 strains resistant to imipenem, 3 preseated with positive IMP gene, 2 presented with positive VIM gene, and 4 presented with positive OXA gene, one out of the 8 strains presented with two genotypes; the DNA sequence of 4OXA gene positive products presented with OXA-23 subtype. Conclusion： The resistance rate to earbopenems of A.baumannii is fairly low in Nanjing, and the resistance in A.baumannii was related to OXA-23, IMP and VIM resistance genes.

关 键 词：鲍曼不动杆菌 耐药性 碳青霉烯酶基因 

分 类 号：Q786[生物学—分子生物学]