宫颈黏膜细胞线粒体DNA核内整合及其与c-myc基因表达的关系  

Relationship between integration of mtDNA fragments in the nuclei of cervical epithelium cells and the expression of c-myc

作　　者：陈道桢[1] 薛文群[1] 龚晓红[1] 吴高雄[1] 项静英[1] 项达军[1] 陆以农[1] 印永祥[1] 

机构地区：[1]南京医科大学附属无锡妇幼保健院妇科,江苏无锡214002

出　　处：《南京医科大学学报（自然科学版）》2006年第10期948-951,F0004,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅重点学科(Z200323);无锡市社会发展课题(HS20030013)资助项目

摘　　要：目的:探讨宫颈黏膜细胞核内线粒体DNA(mtDNA)整合与c-myc基因表达的关系。方法:采用免疫组化(IHC)检测c-myc基因表达;采用原位杂交方法对宫颈黏膜细胞核内mtDNA序列进行检测。结果:宫颈癌黏膜细胞核内mtDNA序列的检出率为27.5%、不典型增生为13.3%、正常宫颈黏膜细胞未发现有mtDNA序列整合。宫颈黏膜细胞c-myc基因表达率在mtDNA序列检出组为67%,显著高于mtDNA序列未检出组(36%)。结论:宫颈黏膜细胞mtDNA序列核内整合可能参与了宫颈癌的发生;c-myc基因表达可能与宫颈黏膜mtDNA序列整合有关。Objective： To explore the relationship between the integration of mtDNA in the nuclei of cervical epithelium cells and the expression of c-myc. Methods：The expression of c-myc proteins was examined in 40 cases of uterine cervix carcinoma and 30 cases of CIN and 30 cases of cervical epithelium; the sequence of mtDNA in the ,nuclei was detected by in situ hybridization. Results：The integration rate of mtDNA in the nuclei was 27.5 % in ce,＇vical carcinoma, 13.3% in CIN, 0% in cervical epithelium. The expression rate of c-myc in integration＊ group （67%）was significantly higher than that in integration- group （36%）： Conclusion： lntegration of mtDNA into ,nuclei of cervical epithelium cells may he involved in the carcinogenesis of cervical epithelium cells and may contribute to the expression of c-myc.

关 键 词：线粒体DNA 整合 原位杂交 c—myc 宫颈癌 

分 类 号：Q786[生物学—分子生物学]