油酸-内毒素序贯致新生大鼠急性肺损伤IL-18水平的变化  

Changes of IL-18 levels in plasma of neonatal rats after sequential injury with oleic acid and lipopolysaccharide

作　　者：史彧[1] 王崇伟[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,南京210008

出　　处：《南京医科大学学报（自然科学版）》2006年第10期941-943,F0003,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨油酸-内毒素序贯致伤新生大鼠的IL-18水平,在急性肺损伤中的作用。方法:采用油酸加内毒素脂多糖腹腔注射新生大鼠致肺损伤模型,在油酸致伤后4h实施内毒素第2次致伤,收集血浆检测IL-18水平,同时设立单剂量内毒素致伤组和生理盐水为对照组,每组12只。结果:油酸-内毒素致伤新生大鼠血浆IL-18水平均显著高于单剂量内毒素致伤组和生理盐水对照组(P<0.01)。结论:油酸-内毒素组IL-18水平显著高于单剂量内毒素致伤组和生理盐水对照组,提示致炎因子IL-18可能在急性肺损伤的发生和发展中起重要的作用。Objective： To study the changes of pro-inflammatory interleukin-18 （IL-18） levels and evaluate the role of IL-18 on acute lung injury （AL1） after sequential injury with oleic acid and lipopolysaecharide. Methods： Enzyme-linked immunosorhent assay （ELISA） was employed to determine the protein concentrations of IL-18 in the plasma of neonatal rats after sequential injury with oleic acid and lipopolysaceharide. Results： The plasma IL-18 level was highest in neonatal rats after sequential injury with oleie acid and lipopolysaecharide than that in neonatal rats hit with lipopolysaccharide（P 〈 0.01 ）. Conclusion： IL-18 is an early proinflammatory cytokine and may play an important role in the development of ALI.

关 键 词：油酸 内毒素 新生大鼠 白细胞介素-18 急性肺损伤 

分 类 号：R722.12[医药卫生—儿科]