巯基乙酸自组装膜DNA电化学传感器对转基因NOS的定量检测  

Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence

作　　者：孙伟[1] 尚智美[1] 杨茂霞[1] 焦奎[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《高等学校化学学报》2006年第10期1859-1861,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20405008和20375020)资助.

摘　　要：A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethyl-aminopropyl） -N＇-ethylcarbodiimide hydrochloride （ EDC ） and N-hydroxysuccinimide （NHS）. By using methylene blue as the electrochemical indicator, the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0 × 10^-8 —1.0 × 10^-4 mol/L with the linear regression equation as△Ipc = 1. 10lgc ＋ 8.25 （ n = 8, γ = 0. 998 5 ） and the detection limit as 3.60 × 10^-8 mol/L（3σ）.

关 键 词：电化学基因传感器 巯基乙酸 NOS基因 亚甲基蓝 自组装膜电极 

分 类 号：O657[理学—分析化学]