高压微波辅助提取法提取牛黄上清丸中的黄芩苷  

High-pressure Microwave-assisted Extraction of Baicalin from Niuhuang Shangqing Pills

作　　者：王玉堂[1] 于永[1] 汪子明[1] 周新[1] 白立飞[1] 丁兰[1] 张寒琦[1] 

机构地区：[1]吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2006年第10期1862-1864,共3页Chemical Journal of Chinese Universities

基　　金：吉林省科技发展计划项目(批准号:20050560)资助.

摘　　要：High-pressure microwave extraction was used to extract the baicalin in Niuhuang Shangqing Pills.According to viscidity of honey pills,the sample was painted on the surface of unshaped glass slide,and then the glass slide was placed in extraction vessel and baicalin in the sample was extracted by microwave radiation.High performance liquid chromatograph(HPLC)was used to determine the content of baicalin.The effects of the microwave extraction pressure and time on the extraction yield of baicalin were also examined.The baicalin in Niuhuang Shangqing Pills obtained from five different factories was determined.The results indicate that the microwave extraction of method B(smearing)is obviously superior to the microwave extraction of method A(slicing),ultrasonic extraction and sexhlet extraction.This method proposed in the paper has advantages such as quickness,convenience and high extraction yield.High-pressure microwave extraction was used to extract the baicalin in Niuhuang Shangqing Pills. According to viscidity of honey pills, the sample was painted on the surface of unshaped glass slide, and then the glass slide was placed in extraction vessel and baicalin in the sample was extracted by microwave radiation. High performance liquid chromatograph （HPLC） was used to determine the content of baicalin. The effects of the microwave extraction pressure and time on the extraction yield of baicalin were also examined. The baicalin in Niuhuang Shangqing Pills obtained from five different factories was determined. The results indicate that the microwave extraction of method B （smearing） is obviously superior to the microwave extraction of method A （ slicing）, ultrasonic extraction and sexhlet extraction. This method proposed in the paper has advantages such as quickness, convenience and high extraction yield.

关 键 词：微波辅助提取 牛黄上清丸 高效液相色谱法 黄芩苷 

分 类 号：O657[理学—分析化学]