结合丝组二肽的树状多肽对λDNA的切割  

Cleavage of λDNA by Seryl-histidine Planted Peptide Dendrimer

作　　者：何骏[1] 麻远[1] 赵玉芬[1] 

机构地区：[1]清华大学化学系

出　　处：《高等学校化学学报》2006年第10期1891-1893,共3页Chemical Journal of Chinese Universities

基　　金：教育部留学回国人员科研启动基金;国家自然科学基金(批准号:20132020)资助.

摘　　要：Artificial nucleic acid cleavage agents have received considerable attention due to their very important applications in biochemistry and molecular biology.Seryl-histidine is a dipeptide possessing DNA,protein and ester cleavage activity.Since peptide dendrimers have a lot of unique physical and chemical properties compared to normal peptide chain and have a great varieties of applications in biological areas,two and four branched seryl-histidine planted peptide dendrimers were synthesized by Fmoc solid phase peptide synthesis approach.The products were purified by HPLC and characterized by ESI-MS.Their DNA cleavage activities were tested by means of Agarose Gel Electrophoresis.In the cleavage reaction,peptide dendrimer showed a strong combining inclination to DNA,highly concentrated dendrimer solution can quench the fluorescence of EB.Even though,the DNA cleavage activity of the dendrimers was lower than that of seryl-histidine dipeptide monomer.Artificial nucleic acid cleavage agents have received considerable attention due to their very important applications in biochemistry and molecular biology. Seryl-histidine is a dipeptide possessing DNA, protein and ester cleavage activity. Since peptide dendrimers have a lot of unique physical and chemical properties compared to normal peptide chain and have a great varieties of applications in biological areas, two and four branched seryl-histidine planted peptide dendrimers were synthesized by Fmoc solid phase peptide synthesis approach. The products were purified by HPLC and characterized by ESI-MS. Their DNA cleavage activities were tested by means of Agarose Gel Electrophoresis. In the cleavage reaction, peptide dendrimer showed a strong combining inclination to DNA, highly concentrated dendrimer solution can quench the fluorescence of EB. Even though, the DNA cleavage activity of the dendrimers was lower than that of seryl-histidine dipeptide monomer.

关 键 词：丝组二肽 树状多肽 DNA切割 

分 类 号：O629.72[理学—有机化学]