魟鱼软骨多糖对小鼠G_(422)胶质瘤血管形成的影响  

Effection of Ray Cartilage Glycosaminoglycans on G_(422) Glioma Angiogenesis

作　　者：罗俊生[1] 刘玉玲[2] 郭斌[1] 

机构地区：[1]锦州医学院附属第一医院,辽宁锦州121001 [2]中国医科大学附属第四医院,辽宁沈阳110001

出　　处：《辽宁中医杂志》2006年第9期1207-1208,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:观察魟鱼软骨多糖对小鼠G422胶质瘤血管形成及瘤组织VEGF mRNA和蛋白表达的影响。方法:建立小鼠G422胶质瘤腋下肿瘤模型,将实验分为生理盐水(Saline)组、不同剂量魟鱼软骨多糖(RCG)组、环磷酰胺(CTX)组,观察肿瘤生长情况,绘制肿瘤生长曲线,CD31免疫组织化学染色计数肿瘤组织微血管密度(M icrovessel density,MVD),采用RT-PCR和W estern b lot技术检测瘤组织血管内皮生长因子(Vascu lar endothelial growth factor,VEGF)mRNA和蛋白表达。结果:魟鱼软骨多糖各剂量组小鼠的肿瘤生长曲线平缓,MVD显著减少,VEGF mRNA和蛋白表达水平显著降低(P<0.01)。结论:魟鱼软骨多糖明显抑制小鼠G422胶质瘤的生长,抑制肿瘤的血管形成,可能与其下调肿瘤VEGF mRNA和蛋白表达有关。Objective ：To explore the effeetion of Ray Cartilage Glycosaminoglycans（ RCG ）on the G422 Glioma angiogenesis and VEGF mRNA and protein expression in G422 Glioma. Methods： The model of mouse with G422 Glioma was made, the experimental mice were randomly divided into normal saline group, RCG groups at varied concentrations and CTX group, Tumor growth states were observed ;Microvessel density （MVD）was quantitated by immunohistochemistry using monoclonal antibodies of CD31 ; The expression of VEGF mRNA and protein in G422 Glioma was determined with RT - PCR and Western blot. Results ：Tumor growth curves in RCG groups were smooth compared with Saline group;there were significant differences of MVD between RCG groups and Saline group ; VEGF mRNA and protein expression levels in RCG groups were reduced significantly compared with Saline group. Conclusions： RCG could effectively inhibite the growth of G422 Glioma and angiogenesis in G422. Glioma,which was probably attributed to reducing the expression of VEGF mRNA and protein.

关 键 词：魟鱼软骨多糖 G422胶质瘤 血管形成 

分 类 号：R286.91[医药卫生—中药学] R979.1[医药卫生—中医学]