钙试剂-蛋白质体系的共振光散射光谱研究  

A study on interaction of calconcarboxylic sodium with proteins by resonance light scattering measurements

作　　者：张姝[1] 李树伟[1] 曾铭[1] 谢永红[1] 

机构地区：[1]四川师范大学化学与材料科学学院,成都610068

出　　处：《分析试验室》2006年第9期30-34,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(50472103)

摘　　要：基于蛋白质对钙试剂共振光散射的增强作用,拟定了一种测定蛋白质的共振光散射法。在pH 4.00的水溶液中,钙试剂在595 nm处的共振光散射增强与蛋白质浓度呈线性关系。此方法对人血清白蛋白、牛血清白蛋白的检出限分别可达0.068和0.085μg/mL。同时得到钙试剂与人血清白蛋白和牛血清白蛋白的结合常数(K)以及结合位点数(n)分别为8.15×104mol/L,0.18和7.67×104mol/L,1.5。The resonance light scattering （RLS） of Calconcarboxylic Sodium （CCS） is greatly enhanced by proteins. Based on this phenomenon, a novel method for the determination of protein by using CCS as a labeling agent was developed. At pH 4.00, the enhanced intensity of RLS at 595 nm is proportional to the concentration of protein. The limit of detection is 0.068 μg/mL for HSA, 0.085 μg/mL for BSA. We obtain the binding constant （K） and binding site number （n） of HSA - CCS are 8.15 × 104 mol/L and 0.18. As for BSA - CCS, the values are 7.67 × 104 mol/L and 1.5, respectively.

关 键 词：共振光散射 钙试剂 人血清白蛋白 牛血清白蛋白 

分 类 号：O657.3[理学—分析化学]