Establishment of genetic transformation system via Agrobacterium in tall fescue cultivar  

作　　者：QIAN Hai-feng Shaukat Ali HONG Liang XU Hao 

机构地区：[1]College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310014 [2]NationalAgricultural Research Centre, Park Road, Islamabad, Pakistan

出　　处：《Journal of Forestry Research》2006年第3期238-242,共5页林业研究（英文版）

基　　金：Foundation project： This paper was supported by Zhejiang Provincial Science and Technology Plan of China （Grant No. 2003C30053） and Zhejiang Provincial Natural Science Foundation of China （Grant No.Y504076）.

摘　　要：Tall fescue （Festuca arundinacea Schreb.） is a cool-season turfgrass used on fairways in golf courses. The object of this study was to develop a more efficient, reliable, and repeatable approach in transforming the grass using Agrobacterium （EHA105）, where β-glucuronidase gene （uidA） was used as a reporter and hygromycin phosphotransferase gene （hyg） as a selectable marker. An effective expression of transgene was observed in transforming 2-month-old calli derived from mature seeds （cv. Bingo） cultured on MS medium supplemented with 9 mg·L^-1 2, 4-D. A two-step solid medium selection with increasing hygromycin concentration （from 30 to 50 mg· L^-1） was used to obtain resistant calli. Transgenic plants have been produced from many independent transformed calli. The presence of functional β-glucuronidase gene （uidA） was detected in hygromycin-resistant calli. Transgenic plants were regenerated and PCR and Southern blot confirmed transgene integration in the tall fescue genome.高教鞭(Festuca arundinacea Schreb ) 是在高尔夫球场在航路上使用的一棵凉爽季节的草地草。这研究的目标是开发更多有效的，可靠，；在用 Agrobacterium (EHA105 ) 转变草重复有能力的途径，在β - glucuronidase 基因(uidA ) 被用作一个记者的地方；hygromycin phosphotransferase 基因(hyg ) 作为一个可选的标记。transgene 的一个有效表达式在转变调用 i 从成熟种子导出的 2-month-old 被观察(cv。宾果游戏) 在与 9 mg · L 补充的 MS 媒介上有教养[1 ] 2， 4-D。有增加 hygromycin 集中的一种二拍子的圆舞固体媒介选择(从 30 ～ 50 mg · L [1 ]) 被用来获得抵抗电话 i。转基因的植物从许多独立转变电话 i 被生产了。功能的β - glucuronidase 基因(uidA ) 的存在在 hygromycin 抵抗的电话 i 被检测。转基因的植物被改革；PCR；南部的污点在高教鞭染色体证实了 transgene 集成。

关 键 词：Tall Fescue  Agrobacterium tumefaciens Transformation β-glucuronidase gene （uidA） 

分 类 号：Q343[生物学—遗传学]