茉莉酸甲酯、水杨酸和一氧化氮诱导怀槐悬浮细胞合成异黄酮及细胞结构变化  

ISOFLAVONE ACCUMULATION ASSOCIATED WITH CELL STRUCTURAL CHANGES IN MAACKIA AMURENSIS SUSPENSION CULTURES ELICITED BY METHYL JASMONATE,SALICYLIC ACID AND NITRIC OXIDE

作　　者：罗建平[1] 夏宁[1] 沈国栋[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《分子细胞生物学报》2006年第5期438-444,共7页Journal of Molecular Cell Biology

基　　金：国家自然科学基金(项目编号:30170059)。~~

摘　　要：本文对比研究了茉莉酸甲酯(MeJA)、水杨酸(SA)和一氧化氮(NO)三种激发子对怀槐悬浮培养物异黄酮合成及细胞结构变化的影响。结果表明,在三种激发子的作用下怀槐细胞异黄酮合成量显著提高:200μmol/L MeJA、100μmol/L SA及50μmol／L SNP处理培养细胞9d后,异黄酮含量分别为同期对照的417．18％、185．45％和222．45％。同时细胞内发现染色很深的电子致密小体(EDB),其数量随着异黄酮含量的升高而增加,亦在第九天达到最多,与异黄酮积累呈现正相关性。推测激发子可能诱导植物细胞结构变化来响应次生代谢产物的合成。Comparison between changes in isoflavone accumulation and cell structure in Maackia amurensis suspension cultures elicited by methyl jasmonate （MeJA）,salicylic acid （SA） and nitric oxide （NO） was studied. The results suggested that MeJA,SA and NO can all stimulate isoflavone production remarkably. After 9d treatment with 200μmol/L MeJA,100μmol/L SA and 50μmol/L SNP,the isoflavone content was 417.18%,185.45% and 222.45% of the control,respectively. At the same time,the electron-dense body （EDB） could be easily found in the cells,while its number achieved most after 9 d treatment,and the number of EDB and isoflavone content were positive correlated. It was deduced that elicitors stimulated plant secondary metabolites production associated with changes in cell structure.

关 键 词：茉莉酸甲酯 水杨酸 一氧化氮 异黄酮 细胞结构 

分 类 号：Q946[生物学—植物学] S567.19[农业科学—中草药栽培]