水稻P0491E01基因调控花药发育的功能分析(简报)  

FUNCTIONAL ANALYSIS OF RICE P0491E01 GENE REGULATING ANTHER DEVELOPMENT

作　　者：俞竹青[1] 朱骏[1] 高菊芳[1] 杨仲南[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《分子细胞生物学报》2006年第5期467-472,共6页Journal of Molecular Cell Biology

基　　金：上海市教育发展基金会;上海市教委曙光计划;科技部973计划(2005CB120802)的资助。

摘　　要：高等植物的花药发育是包含基因不同程度相互作用的复杂发育过程，一般可分为两个阶段。第一个是花药的形态建成阶段，在这一阶段中细胞与组织发生分化，小孢子母细胞进行减数分裂形成四分体小孢子。第二个是花粉的形成和成熟阶段，在这一阶段细胞开始衰退、凋亡，小孢子进行有丝分裂、分化发育成成熟花粉，花药膨大并被花丝托到合适的位置．组织发生衰退开裂，花粉囊破裂，花粉粒释放。在整个花药发育过程中有大量的基因在花药各组织中表达，据估计仅特异性表达的基因就有3500个左右．而其中60—100个基因为花粉发育所必需的．这其中任何一个基因的突变都会导致雄性不育。The rice P0491E01 gene shares high similarity in amino acid sequence with A rabidapsis gene AtDAD1 （DEFECTIVE IN ANTHER DEHISCENCE1）which plays a key role in the biosynthesis of jasmonic acid. In this paper, we investigated the function of this gene in the anther development of rice using RNA interference strategy. An exon fragment of 263bp was cloned from genomic DNA of P0491E01 to construct RNAi vector pP0491RNAi. Then, pP0491RNAi was transformed into O.sativa japonica by Agrobacterium-mediated transformation and ten transgenic plants were obtained. GUS-staining and PCR analysis confirmed that T-DNA was integrated into rice genome. Three of the transgenic plants were male sterile, and the other transgenic plants showed reduced fertility. Cytological observation indicated that anther development in the early stage of male sterile transgenic plants was normal, however, the microspores could not develop into mature pollen grains. Further investigations of the expression of P0491E01 gene in these transgenic lines by RTPCR revealed that its transcription was significantly reduced. The results suggest that P0491E01 may play an important role during the late stage of anther development.

关 键 词：水稻 花药发育 RNA干涉 RT—PCR 

分 类 号：S511[农业科学—作物学] Q943[生物学—植物学]