分离MEF和ES细胞的差速贴壁法研究(简报)  

THE STUDY OF SEPARATING MURINE EMBRYONIC FIBROBLAST FROM EMBRYONIC STEM CELLS BY THE DIFFERENTIAL ADHESION METHOD

作　　者：徐超[1] 黄华荣[1] 俞宏[1] 赵小立[1] 陆永良[2] 戴利成[2] 姚行[2] 

机构地区：[1]浙江大学生命科学学院,杭州310012 [2]浙江省湖州市中心医院,湖州313000

出　　处：《分子细胞生物学报》2006年第5期477-481,共5页Journal of Molecular Cell Biology

基　　金：浙江省重大项目(J20020579-30116);湖州中心医院合作项目(H20010984-32536)

摘　　要：胚胎干细胞的分化控制是胚胎干细胞研究的一个重要方面。由于常规的拟胚体诱导途径是在形成拟胚体后才开始进行诱导分化．受多种胚层的共同作用使得我们无法简便探索诱导分化的机制，而且用这种方法进行的诱导分化试验的结果检测比较困难且不够精确，因此通过单层贴壁诱导途径进行胚胎干细胞的分化研究的报道越来越多。Ying QL and Takahashi T．等使用了一种不经过拟胚体途径的单层ES细胞培养方法，不仅简化了操作步骤，而且能够精确观察到诱导分化的过程。This study has established a simple method of separating murine embryonic fibroblasts （MEF） from embryonic stem cells （ES cells）. The adhesion rates of MEF and ES cells were compared to find out the opitimum time of removing MEF . The result shows that 1.5h and 0.5% gelatin concentration is the opitimum condition of removing MEF. Furthermore,the ES cells which have been purified have the same plating efficiency and the ability of three germ layers differentiation as the unpurified ES cells. After differential adhension,there is strong ALP activity in ES cells. All the results show that the purified ES cells are still in the state of undifferentiation and maintain the pluripotent.

关 键 词：胚胎干细胞 饲养层细胞 差速贴壁 拟胚体 

分 类 号：Q813[生物学—生物工程]