生物衍生骨-WO-1药物缓释系统结构特征和体外药物释放的研究  

STUDIES OF PREPARATION, MORPHOLOGY AND IN VITRO RELEASE ON BIO-DERIVED MATERIAL-WO-1 DELIVERY SYSTEM

作　　者：任永信[1,2] 杨志明[1] 解慧琪[1] 秦廷武[1] 李秀群[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室.干细胞与组织工程研究室,成都610041 [2]现在南京医科大学第一附属医院骨科

出　　处：《中国修复重建外科杂志》2006年第10期1044-1046,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划(863)资助项目(2002AA205011)~~

摘　　要：目的制备生物衍生骨WO-1药物缓释系统，对其理化性质和WO-1的体外释放情况进行评价。方法采用Pluronic F-127负载WO-1制作生物衍生骨WO-1药物缓释系统。扫描电镜观察生物衍生骨及生物衍生骨WO-1药物缓释系统形貌特征，测量其孔径和孔隙率；应用x线衍射分析仪和x线能量散射分析仪对其成分进行分析；应用高效液相色谱分析仪评价WO-1在双蒸水中1～7d的药物释放情况。结果生物衍生骨具有天然的网状孔隙结构，孔隙间互相连通；生物衍生骨-WO1药物缓释系统也具有互相连通的网状孔隙结构，孔径和孔隙率分别为522．43±16．75μm和55％，低于生物衍生骨的孔径623．67±12．31μm和孔隙率75％。生物衍生骨一WO-1药物缓释系统主要成分为羟基磷灰石，钙／磷比为1．54；生物衍生骨钙／磷比为1．77。药物释放实验：生物衍生骨-WO1药物缓释系统1d表现为爆发释放，溶液浓度为4．6／μg／ml，此后可连续释放6d，浓度维持在0．2～0．8μg／ml。结论生物衍生骨-WO-1药物缓释系统可望成为一种更佳的骨组织工程支架材料，有待于进一步进行体内研究。Objective To study the potential of a bio-derived material combined with Pluronic F-127 in vitro as a delivery vehicle for WO 1 in the bone repair therapy. Methods Bio-derived materials were fabricated and loaded with WO-1 by Pluronic F-127. Micromorphology and porosity were detected by the scanning electron microscope and the digital image analysis system respectively. The WO 1 release from the system in vitro was studied by the high performance liquid chromatography. Results Bio-derived material-WO 1 drug delivery systems were created with the interconnected pore network. The porosity and pore size of the system were 55% and 522.43±16.75 μm respectively, compared with those of bio-derived materials, which were 75% and 623. 67± 12. 31μm respectively. And the main composition of the system was HA. The in vitro release kinetics of WO-1 revealed that an effective therapeutic concentration（0. 2-0. 8 μg/ml） of WO-1 was maintained for 6 days after a high initial burst release. Conclusion The bio-derived material-WO 1 drug delivery system can be used in the bone repair therapy. However, the in vivo study on it is still needed.

关 键 词：组织工程骨 生物衍生骨 药物缓释 WO-1 

分 类 号：R318[医药卫生—生物医学工程] Q813[医药卫生—基础医学]