Sphingosylphosphorylcholine stimulates human monocyte-derived dendritic cell chemotaxis  

作　　者：Ha-young LEE Eun-ha SHIN Yoe-sik BAE 

机构地区：[1]Medical Research Center for Cancer Molecular Therapy, College of Medicine, Dong-A University, Busan 602-714, Korea [2]Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Korea

出　　处：《Acta Pharmacologica Sinica》2006年第10期1359-1366,共8页中国药理学报（英文版）

摘　　要：Aim： To investigate the effects of sphingosylphosphorylcholine （SPC） on human monocyte-derived dendritic cell （DC） chemotaxis. Methods： Human DC were generated from peripheral blood monocytes by culturing them with granulocyte macrophage-colony stimulating factor and interleukin-4. The effect of SPC on the DC chemotactic migration was measured by chemotaxis assay. Intracellular signaling event involved in the SPC-induced DC chemotaxis was investigated with several inhibitors for specific kinase. The expression of the SPC receptors was examined by reverse transcription polymerase chain reaction. Results： We found that SPC induced chemotactic migration in immature DC （iDC） and mature DC （mDC）. In terms of SPC-induced signaling events, mitogen activated protein kinase activation and Akt activation in iDC and mDC were stimulated. SPC-induced chemotaxis was mediated by extracellular signal-regulated protein kinase and phosphoinositide-3-kinase, but not by calcium in both iDC and mDC. Although mDC express ovarian cancer G protein-coupled receptor 1, but not G protein-coupled receptor 4, iDC do not express any of these receptors. To examine the involvement of sphingosine-1-phosphate （SIP） receptors, we checked the effect of an SIP receptor antagonist （VPC23019） on SPC-induced DC chemotaxis. VPC23019 did not affect SPC-induced DC chemotaxis. Conclusion： The results suggest that SPC may play a role in regulating DC trafficking during phagocytosis and the T cell-stimulating phase, and the unique SPC receptor, which is different from S l P receptors, is involved in SPC-induced chemotaxis.Aim： To investigate the effects of sphingosylphosphorylcholine （SPC） on human monocyte-derived dendritic cell （DC） chemotaxis. Methods： Human DC were generated from peripheral blood monocytes by culturing them with granulocyte macrophage-colony stimulating factor and interleukin-4. The effect of SPC on the DC chemotactic migration was measured by chemotaxis assay. Intracellular signaling event involved in the SPC-induced DC chemotaxis was investigated with several inhibitors for specific kinase. The expression of the SPC receptors was examined by reverse transcription polymerase chain reaction. Results： We found that SPC induced chemotactic migration in immature DC （iDC） and mature DC （mDC）. In terms of SPC-induced signaling events, mitogen activated protein kinase activation and Akt activation in iDC and mDC were stimulated. SPC-induced chemotaxis was mediated by extracellular signal-regulated protein kinase and phosphoinositide-3-kinase, but not by calcium in both iDC and mDC. Although mDC express ovarian cancer G protein-coupled receptor 1, but not G protein-coupled receptor 4, iDC do not express any of these receptors. To examine the involvement of sphingosine-1-phosphate （SIP） receptors, we checked the effect of an SIP receptor antagonist （VPC23019） on SPC-induced DC chemotaxis. VPC23019 did not affect SPC-induced DC chemotaxis. Conclusion： The results suggest that SPC may play a role in regulating DC trafficking during phagocytosis and the T cell-stimulating phase, and the unique SPC receptor, which is different from S l P receptors, is involved in SPC-induced chemotaxis.

关 键 词：dendritic cells sphingosylphosphorylcho line CHEMOTAXIS pertussis toxin-sensitive G protein extracellular signal regulated protein kinase 

分 类 号：R516.6[医药卫生—内科学]