高温下L-精氨酸对脂质过氧化及细胞膜影响  

Effect of L-Arg on lipid peroxidation and cellular membrane lipid fluidity subjected under heat stresses in mice

作　　者：罗海吉[1] 孔亮[1] 周爱军[1] 卢晓翠[1] 杨真[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院营养与食品卫生学系,广州510515

出　　处：《中国公共卫生》2006年第10期1231-1233,共3页Chinese Journal of Public Health

摘　　要：目的探讨L-精氨酸(L-Arg)对高温下脂质过氧化及细胞膜脂流动性的影响。方法采用三因素析因设计,将SD雄性大鼠随机分成3组:A(灌水对照组),B(补充L-Arg组),C〔维生素E(VE)组〕,饲养12 d。取1和2 h热应激时间,在热应激前、后不同的时间点采样。测定血清超氧化物岐化酶(SOD)、血清丙二醛(MDA);细胞膜脂流动性检测采用激光扫描共聚焦显微镜。结果热应激后各组SOD活性均较相应常温对照组明显升高,而B组和C组又明显高于A组(P<0.05)。各组血清MDA含量明显高于相应的常温对照组(P<0.05),且随着热应激时间的增加,B组和C组血清DMA含量低于A组(P<0.05)。热应激后B组和C组巨噬细胞膜脂流动性明显升高,并且高于A组(P<0.05)。结论适量补充L-Arg能增强SOD活性,减少MDA含量,有效地保持细胞膜脂流动性,抑制脂质过氧化损伤。L-Arg具有与VE同样的抗氧化能力可能与其提高SOD活性及通过L-Arg-NO途径、参与氧化应激调节有关。Objective To explore the effect of L- Arg on mice lipid peroxidation and cellular membrane lipid fluidity subjected under high temperature. Methods Male adult SD mice were randomly divided into three groups, fed 12 days, exposed under heat stresses, sampled at different hours through heat stress. The activity of superoxide dismutase（SOD） in serum and the yield of malondialdehyde（MDA） were measured with kit. Using Laser Scanning Cortfoeal Microscopy（LSCM） and linearity quantitation scanning to detect the cellular membrane lipid fluidity. Results After heat stress SOD of all groups were higher than control group, group B and group C were obviously higher than group A（（P〈0.05）.The yield of MDA had stepped up than before, By the time adding, MDA of group B and group C were lower than group A（ P 〈 0.05 ）. Cellular membrane lipid fluidity group B and group C after heat stress were higher than before, and it were also higher than group A under heat stress（P 〈 0.05 ）. Conclusion Appropriate L - Arg suplement could increase the activity of SOD in serum, decrease the yield of MDA, keep the cellular membrane lipid fluidity efficiendy, in order to inhibit injury of lipid peroxidation. L - Arg has the same antioxidation as VE, It is presumed that possibly throught increasing the activity of SOD and by L - Arg - NO pathway.

关 键 词：精氨酸(L-Arg) 热应激 脂质过氧化 细胞膜脂流动性 

分 类 号：R135.3[医药卫生—劳动卫生]