上皮钙粘素基因外显子13C/T单核苷酸多态性与膀胱移行细胞癌的关系  

A correlation study of Single Nucleotide Polymorphisms in Exon 13 of E-cadherin gene CDH1 with transitional cell carcinoma of the bladder

作　　者：郑涛[1] 张旭[2] 马鑫[2] 李宏召[3] 张军[2] 傅斌[2] 郎斌[2] 许凯[2] 

机构地区：[1]襄樊市中心医院泌尿外科,湖北襄樊441021 [2]华中科技大学同济医学院附属同济医院泌尿外科 [3]中南大学湘雅医院泌尿外科

出　　处：《临床泌尿外科杂志》2006年第10期776-778,781,共4页Journal of Clinical Urology

基　　金：国家自然科学基金资助项目(No.30471737)

摘　　要：目的:探讨上皮钙粘素(E-cad)外显子13(Exon-13)C/T单核苷酸多态性与膀胱移行细胞癌的生物学行为之间的关系。方法:对膀胱移行细胞癌患者130例(膀胱癌组)和健康人及非肿瘤患者60例(对照组),采用聚合酶链反应和DNA测序方法,检测包含上皮钙粘素基因Exon-13的DNA片段的基因序列,分析该片段中的SNP。选择χ2检验和t检验进行差异显著性分析,Woolf法计算OR值和95%CI。结果:膀胱癌组E-cad基因Exon-13154bp处T等位基因频率高于对照组(P<0.01);膀胱癌组中浸润性膀胱癌T等位基因频率高于表浅膀胱癌(P<0.05);膀胱移行细胞癌病理分化程度分级与T等位基因频率之间呈正相关(P<0.05)。结论:E-cad基因Exon-13154处T等位基因频率与膀胱移行细胞癌的发生及其侵袭能力密切相关。Objective： To investigate whether single nucleotide polymorphisms in Exon 13 of E cadherin gene are associated with transitional cell carcinoma of urinary bladder （TCCB）. Methods： A hospital-based case-control study was performed on 130 patients with TCCB （male 96 and female 34, age form 29 to 84, mean （58.1±14.7） and 60 normal controls （male 31 and female 19, age form 28 to 81, mean （56.1±14.8）. Genotypes were determined using DNA sequencing. SNP in E-cadherin DNA fragments of Exon-13 were analyzed. Cignificance of difference was determined by Chi square test and Studenfs t-test. Calculation of OR and 95%CI was performed by Woolf method. Results：The T allele frequencies at 154 position of E-cadherin gene Exon-13 were significantly higher in case group than in control group （P〈0.01）. The T allele frequencies in superficial TCCB patients were high er than those in invasive TCCB patients （P〈0.05）. Conclusions：The T allele frequencies at 154 position of E-cad- herin gene Exon-13 were associated with carcinogenesis and invasive capability of TCCB.

关 键 词：肿瘤标记物 膀胱肿瘤 生物学行为 

分 类 号：R737.14[医药卫生—肿瘤]