靶向STAT3 decoy寡核苷酸对SLE患者PBMC中IFI16表达的影响及其意义  

The effect of STAT3 decoy oligodeoxynucleotides on the expression of IFI16 in peripheral blood mononuclear cell of patients with systemic lupus erythematosus

作　　者：徐祖森[1] 周映[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院皮肤科,武汉430030

出　　处：《中华风湿病学杂志》2006年第10期599-602,共4页Chinese Journal of Rheumatology

摘　　要：目的观察特异性靶向信号转导和转录激活子3(STAT3)的decoy寡核苷酸(ODNs)对系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中干扰素诱导核蛋白16(IFI16)表达水平的影响。方法通过体外细胞培养术,将STAT3 decoy ODNs转染入提取的PBMC内,转染前后分别用反转录聚合酶链反应(RT-PCR)检测PBMC中IFI16的表达水平。结果转染STAT3 decoy ODNs前,IFll6 mRNA的表达水平在活动期、非活动期组与对照组比较明显增高,差异均有统计学意义(P＜0．05)。而活动期与非活动期组比较,差异无统计学意义(P＞0．05)。转染后,IFI16 mRNA的表达水平在活动期与非活动期组中均明显下降,与阴性对照比较差异均有统计学意义(P＜0．05),而正常组转染后与阴性对照比较差异无统计学意义(P＞0．05)。结论IFl16在SLE患者的异常高表达提示IFI16参与SLE的发病。SLE患者异常高表达的IFI16基因能被STAT3 decoy ODNs高度抑制,而在正常人不能被明显抑制,提示SLE患者异常高表达的IFI16是受JAK-STAT3信号转导途径调控的。Objective To investigate the effect of signal transducer and activator of transcription 3 （STAT3） decoy oligodeoxynucleotides （ODNs） on the expression of IFI16 in the peripheral blood mononuclear cell of patients with systemic lupus erythematosus （SLE）. Methods STAT3 decoy ODNs was transfected to PBMC in vitro, and RT-PCR was used to semiquantitatively analyze the IFI16 mRNA in the peripheral blood mononuclear cell （PBMC） before and after the transfection. Results The mean level of IFI16 mRNA in active SLE group and inactive SLE group were much higher than that of normal control. The difference was significant （P〈0.05）. However, there was no statistical difference between active and inactive SLE group （P〉 0.05）. After being transfected by STAT3 decoy ODNs, the level of IFI16 mRNA in active and inactive SLE group were markedly decreased comparing with negative controls. The difference was significant （P〈0.05）. However, the difference was not significant in normal control （P〉O.05）. Conclusions The abnormal overex- pression of IFI16 mRNA in PBMC of patients with SLE suggests that IFI16 may contribute to the pathogenesis of SLE and the overexpression of IFI16 may be mediated through JAK-STAT3 pathway.

关 键 词：红斑狼疮 系统性 核蛋白类 信号转导和转录激活子3 DECOY寡核苷酸 

分 类 号：R593.241[医药卫生—内科学]