牡丹皮多糖2b对高糖所致大鼠系膜细胞增生及活性氧产生的影响  

Effect of Polysaccharides-2b from Mudan Cortex on High Glucose Induced Proliferation of Rat Mesangial Cells and Reactive Oxygen Species in Culture

作　　者：杨波[1] 沈业寿[1] 赵帜平[1] 

机构地区：[1]安徽大学糖复合物与糖工程实验室安徽省生态工程与生物技术重点实验室,安徽合肥230039

出　　处：《中国中医药信息杂志》2006年第10期40-41,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：安徽省十五重大科技专项基金(01803011)

摘　　要：目的探讨高糖对大鼠系膜细胞(MC)增生及活性氧产生的影响,以及牡丹皮多糖2b(PSM2b)对其的干预作用。方法采用MTT法和比色法,分别检测不同浓度PSM2b各组体外高糖培养的MC增生和细胞上清液中超氧化物歧化酶(SOD)的活力和丙二醛(MDA)的水平。结果PSM2b对高糖所致的MC增殖有显著的抑制作用,其抑制作用呈浓度依赖性,并且能够提高上清液中SOD活力,降低MDA含量。结论高糖可导致MC增生和活性氧产生增多,而PSM2b能进行有效的干预。Objective To investigate the effect of high glucose on proliferation of rat mesangial cells and reactive oxygen species （ROS） in culture and the intervention of polysaccharides-2b from Mudan Cortex （PSM2b）. Methods Cell proliferation was assessed by MTT colorirnetric assay. Colorimetric assay was used to detect SOD and MDA in the supematant of high glucose culture of rat mesangial cells. Results PSM2b could significantly inhibit the proliferation of mesangial cells, the inhibiting effect showed a dose-dependent manner. And SOD increased with PSM2b compared with that only with high glucose, while MDA decreased. Condwion High glucose contributes to an increase in rat mesangial cells and ROS produced by rat mesangial cells and PSM2b can better control high glucose-induced proliferation of rat mesanglal cells and oxidative stress.

关 键 词：牡丹皮多糖2b 系膜细胞 高糖 活性氧 

分 类 号：R285.5[医药卫生—中药学]