用PCR扩增16S rDNA检测泡桐组培苗丛枝病类菌原体  

DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA

作　　者：李江山[1] 金开璇 汪跃 熊耀国[1] 邱并生[3] 

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]中国林业研究院森林保护研究所,北京100091 [3]中国科学院微生物研究所,北京100080

出　　处：《林业科学》1996年第6期569-573,共5页Scientia Silvae Sinicae

基　　金：八.五攻关课题!"泡桐丛枝病防治技术的研究";"泡桐胶合板材优良无性系选育"

摘　　要：An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches’ broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1. 2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownlh spp., but no l. 2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9. 4pg of total DNA from infected Paulernia tissue culture plantlets.An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1. 2kb fragment DNA from Paulownia Witches' broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1. 2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownlh spp., but no l. 2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9. 4pg of total DNA from infected Paulernia tissue culture plantlets.

关 键 词：泡桐 丛枝病 类菌原体 

分 类 号：S763.724.3[农业科学—森林保护学]