脯氨酸和硝酸银对甜玉米幼胚愈伤组织诱导与分化的影响  

Influence of L-Pro and AgNO_3 on Callus Inducing and Differentiation in Sweet Corn

作　　者：窦秉德[1] 张永霞[1] 陈莉[1] 

机构地区：[1]淮阴师范学院生物系

出　　处：《淮阴师范学院学报（自然科学版）》2006年第3期233-237,共5页Journal of Huaiyin Teachers College;Natural Science Edition

基　　金：江苏省高校高新技术产业化资助项目(JH03-003)

摘　　要：以甜玉米TD08、TD10的幼胚为外植体,利用不同培养基对其幼胚进行愈伤组织诱导,着重探讨脯氨酸对玉米愈伤组织诱导的影响,同时检验硝酸银是否能替代脯氨酸在玉米愈伤组织诱导中所起的作用.结果表明,脯氨酸有利于玉米愈伤组织诱导且愈伤质量较好;硝酸银不能替代脯氨酸在玉米愈伤组织诱导及分化中所起的作用.To test the function of different ingredient in cultural medium, the young embryos of sweet corn TD08 and TD10 were used as explant in this cultural experiment, mainly concerning the L-Pro influence on the callus inducing and differentiation. The results indicated that L-Pro was fit to add in cultural medium to get a high rote and good quality of callus, AgNO3 was not suitable to substitute the function of L-Pro in callus inducing and differentiation.

关 键 词：甜玉米 幼胚 培养基 愈伤组织 诱导率 

分 类 号：S336[农业科学—作物遗传育种] S513[农业科学—农艺学]