细胞因子诱导破骨细胞分化的2种实验模型的建立  

Establishment of two experimental models of osteoclast differentiation induced by cytokines

作　　者：蒋东芳[1] 吕厚山[1] 林剑浩[1] 姜军[1] 陈占昆[1] 

机构地区：[1]北京大学人民医院关节病诊疗中心,北京100044

出　　处：《基础医学与临床》2006年第9期1008-1012,共5页Basic and Clinical Medicine

摘　　要：目的探讨体外从单个核细胞诱导分化形成破骨细胞的2种不同方法,建立研究细胞因子对破骨细胞分化作用的2种不同实验模型。方法外周血单个核细胞在10-8mol/L LTB4和25μg/LM-CSF刺激下,经过2周的直接诱导分化;外周血单个核细胞与RAFLs共培养,加入10-8mol/L LTB4和25μg/L M-CSF,经过3周的间接诱导分化,用抗酒石酸酸性磷酸酶(TRAP)染色鉴定破骨细胞样细胞(OCL)。结果通过直接分化和间接分化均可形成破骨细胞样细胞。结论2种不同实验模型能分别用于研究细胞因子对破骨细胞的直接和间接分化作用。Objective Establishment of two experimental models for osteoclast differentiation from monocyte in vitro, and to study the potential of osteoclast differentiation induced by cytokines. Methods Direct model of osteoclast differentiation： CD14 ＋ monocyte fraction of peripheral blood mononuclear cell （PBMC） stimulated by 25 μg/L M-CSF ＋ 10^-8 mol/L LTB4 for two weeks. Indirect model of osteoclast differentiation： Utilize the coculture model of RAFLs and monocyte that were stimulated in the presence of 25 g/L M-CSF ＋ 10^-8 -Smol/L LTB4 for three weeks. In TRAP staining the muhinucleated TRAP staining positive osteoclast-like cells were counted as marker of as differentiation effect of each group. Results Osteoclast-like cells can be induced by both direct and indirect models. Conclusion Two experimental models for osteoclast differentiation can be separately used to study the effect of various cytokines for direct and indirect OC differentiation.

关 键 词：单核细胞 破骨细胞 细胞因子 实验模型 

分 类 号：Q254[生物学—细胞生物学]