中药调肝理脾方对大鼠乙醇性肝病肝纤维化的影响  

Effect of Tiaogan lipi decoction on alcoholic liver disease in rats

作　　者：徐光福[1] 褚月虎[1] 王晓阳[1] 田德禄[2] 王新月[2] 李澎涛[3] 杨晋翔[2] 姜良铎[2] 

机构地区：[1]北京中医药大学附属东直门医院感染疾病科,北京市100700 [2]北京中医药大学附属东直门医院消化内科,北京市100700 [3]北京中医药大学基础医学院,北京市100029

出　　处：《世界华人消化杂志》2006年第24期2386-2394,共9页World Chinese Journal of Digestology

摘　　要：目的:研讨中药调肝理脾方和东宝肝泰片对乙醇性肝病肝纤维化的治疗效果。方法:♂SD大鼠经过9 wk乙醇混合物灌胃后,模型组被处死,取得肝脏,其他动物随机分成自然恢复组(11 wk)、东宝肝泰片组和调肝理脾方组,给予连续10 d的灌胃治疗,然后处死并取得肝脏,采用光镜、电镜观察肝细胞、肝星状细胞、肝窦内皮细胞、肝窦和窦周病理变化,并以免疫组化方法观察3种细胞外基质分子变化．结果:模型组动物肝细胞混浊肿胀,脂肪变性,间质细胞增生,细胞核深染,Masson染色并可见肝细胞周围铁丝网格样纤维化和中央静脉基底膜增厚;自然恢复组肝细胞病变有所好转,纤维化也有好转,调肝理脾方组好转最明显．电镜下显示,模型组肝窦基底膜增厚,窦周胶原原纤维大量增生,肝细胞微绒毛大量减少、倒伏,自然恢复组无明显好转,调肝理脾方和东宝肝泰片片组有好转．Ⅰ型胶原和层黏蛋白恢复结果以调肝理脾方组最好,Ⅳ型胶原恢复结果以东宝肝泰片组最好．结论:调肝理脾方对病理性堆积的Ⅰ型胶原和层黏蛋白代谢有明显促进作用,效果强于东宝肝泰片,而且也能促进过多的Ⅳ型胶原恢复正常,而东宝肝泰片在促进堆积的Ⅳ型胶原恢复方面强于调肝理脾方．AIM： To investigate the effects of Tiangan Lipi decoction （TLD） and Dongbao Gantai tablets （DGT） on the alcoholic liver diseases in rats. METHODS： The male SD rats in model group were decollated and the livers were harvested after being fed with a mixture of alcohol for 63 d, and the others were randomly divided into natural recovery （NR） group （11 wk）, TLD treatment group and DGT treatment group. After lavage treatment for 10 d, all the rats were executed and livers were harvested for observation of pathological changes of hepatocytes, hepatic stellate cells, sinusoidal endothelial cells, sinus, and peri-sinus, respectively, under light and electron microscope, and three kinds of extracellular matrix （ECM） generation were also detected by immunohistochemistry. RESULTS： The hepatocytes were tumid and turbid, with fatty denaturalization, and the hyperplasia of stromal cells appeared in model rats. Wire netting around hepatocytes and the incrassation of basilar membrane of central vein were clearly observed by Masson staining. The pathological changes, such as fibrosis, in NR group alleviated to some extent, and the changes recovered the most in TLD group. The incrassation of basilar membrane of sinus, with a great amount of fibril around, were found in model rats by electron microscopy. Microvilli of hepatocytes disappeared or fell down in large numbers, but changes of microstructures were markedly mitigated in DGT and TLD group in comparison with those in NR group. Collagen Ⅰ and Laminin in TLD group had a better recovery than those in DGT and NRG group, while the recovery of collagen Ⅳ was the best in DGT group CONCLUSION： TLD is superior to DGT on the reversion of pathologically accumulated collagen Ⅰ and Laminin, while DGT is superior to TLD on promoting the metabolism of accumulated collagen Ⅳ.

关 键 词：乙醇性肝病 肝窦毛细血管化 窦周纤维化 调肝理脾方 东宝肝泰片 

分 类 号：R259.752[医药卫生—中西医结合]