电针足三里穴抗大鼠应激性胃黏膜损伤作用与NTS内受体的关系  

Effect of electroacupuncturing Zusanli point on stress-induced gastric mucosal injury and and its relationship with receptors in nucleus tractus solitarius

作　　者：黄碧兰[1] 王秋桂[2] 余良主[1] 唐琼[1] 丁洁琼[1] 

机构地区：[1]咸宁学院医学院生理学教研室,湖北省咸宁市437100 [2]咸宁学院医学院组织胚胎学教研室,湖北省咸宁市437100

出　　处：《世界华人消化杂志》2006年第24期2444-2447,共4页World Chinese Journal of Digestology

基　　金：院科研基金资助项目;No.KY0539~~

摘　　要：目的:探讨(EA)足三里穴抗大鼠应激性胃黏膜损伤作用与NTS(NTS)内受体关系．方法:健康♂SD大白鼠56只随机分为8组:应激模型组、EA-应激组、生理盐水(NS)-EA-应激组、哌唑嗪-EA-应激组、育亨宾-EA-应激组、心得安-EA-应激组、阿托品-EA-应激组、纳络酮-EA-应激组．采用束缚-冷方法制备大鼠应激性胃溃疡模型,观察NTS内微量注入不同受体阻断剂,胃溃疡指数(UI)的变化．结果:NS-EA-应激组、EA-应激组大鼠胃黏膜损伤明显减轻,UI分别为26．3±3．5,25．4±3．1,与应激模型组37．5±4．2比较有显著性差异(t= 5．42,t=6．13,P<0．01)．哌唑嗪-EA-应激组UI为34．6±3．4明显高于NS-EA-应激组(t=4．50, P<0．01)．育亨宾-EA-应激组和心得安-EA-应激组分别与NS-EA-应激组比较,UI无显著性差异．阿托品-EA-应激组大鼠UI为33．1±3．7明显高于NS-EA-应激组(t=3．53,P<0．01),纳络酮-EA-应激组与NS-EA-应激组比较,UI无显著性意义．结论:EA对大鼠应激性胃黏膜损伤的保护作用部分是通过NTS内α1,M受体介导的,而与α2,β和阿片肽受体无明显关系．AIM： To investigate the relationship between the effect of electroacupuncturing （EA） Zusanli point on the stress-induced gastric mucosal injury and the receptors in nucleus tractus solitarius （NTS） in rats. METHODS： Fifty-six male SD rats were randomly divided into 8 groups, and gastric ulcer was induced in all the rats by constraint cold method. Except those in model group, the rats were treated with EA, NS＋EA, prazisin＋EA, yohimbine＋EA, propranolol＋EA, atropine＋EA, and naloxone＋EA, respectively. The ulcer indexes were observed after microinjection of various receptor antagonists into NTS.RESULTS： In comparison with that in model group, the UI was significantly reduced in NS＋EA or EA group （26.3 ± 3.5, 25.4 ± 3.1 vs 37.5 ± 4.2, t = 5.42, t = 6.13, both P 〈 0.01）. In comparison with that NS＋EA stree group, the UI was markedly increased in prazisin＋EA or tropine＋EA group （34.6 ± 3.4, 33.1 ± 3.7 vs 26.3 ± 3.5, t = 4.50, t = 3.53, both P 〈 0.01）, but it was not notably different from that in yohimbine＋EA, propranolo＋EA and naloxone-EA group. CONCLUSION： Electroacupuncture plays a protective role in gastric mucosa against stressinduced injury in rats, which is partly mediated by α1 and M type receptors, but not related to α2, β and opioid receptors.

关 键 词：NTS 受体阻断剂 应激性胃黏膜损伤 

分 类 号：R245[医药卫生—针灸推拿学]