人类NRAMP1基因单核苷酸多态与接尘工人肺结核易感性  

Relationship between single nucleotide polymorphisms of NRAMP1 gene and susceptibility to pulmonary tuberculosis in workers exposed to silica dusts

作　　者：曲亚斌[1] 唐云霞[2] 张忠彬[1] 朱人[1] 刘静[1] 顾寿永[1] 卢国良[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生学教研室,上海200032 [2]马鞍山市中心医院职业病科

出　　处：《中华劳动卫生职业病杂志》2006年第9期531-533,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨人类自然抵抗相关巨噬细胞蛋白1(NRAMP1)基因多态性与接尘工人肺结核易感性的关系。方法采用1∶2病例对照设计,按年龄相差小于5岁,工种、吸烟、饮酒率、总粉尘接触量和矽肺患病同比例匹配,选择61例男性肺结核患者为病例组(矽肺50例、非矽肺11例),122例男性无肺结核者为对照组(矽肺100例、非矽肺22例)。应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术检测NRANP1 INT4和D543N位点的多态性。结果NRAMP1 INT4多态位点野生纯合子(G/G)、杂合子(G/C)和突变纯合子(C/C)在病例组的分布频率分别为63．9%、34．4%、1．6%,与对照组比较,差异有统计学意义(P＜0．05),NRAMP1 INT4 C等位基因携带者患肺结核的危险性升高(OR=2．73,95%CI∶1．32～5．64),D543N位点多态与接尘工人肺结核易感性之间无关联(P＞0．05)。结论NRAMP1基因第4内含子G＞C单核苷酸可能是接尘工人肺结核的易感因素。Objective To explore the relationship between polymorphisms of natural resistance-associated macrophage protein 1 （NRAMP1） gene and genetic susceptibility of pulmonary tuberculosis（PTB） in workers exposed to silica dusts. Methods A 1：2 case control study of 61 male workers with PTB（50 silicosis patients and 11 unsilicosis workers） as the case group and 122 male PTB-free workers（100 silicosis patients and 22 unsilicosis workers） as the control group was conducted with the frequency matched for age of ＋ 5 years, the job, the silica exposure, and the condition of cigarette smoking and alcohol drinking. The polymerase chain reaction-restrained fragment length polymorphism technique（PCR-RFLP） was used to detect the single nucleotide polymorphisms（SNPs） of NRAMP1 INT4 and D543N. Results There was a 2.73 times（95 % CI：1.32 - 5.64） increased risk of silicosis for individuals with C allele of NRAMP1 INT4 compared with individuals carrying homozygote（G/G）, while SNPs of NRAMP1 D543N was not associated with FIB （ P 〉 0.05）. Condusion The G 〉 C mutation of intron 4 of NRAMP1 gene might be a susceptible factor of silica for the workers exposed to FIB.

关 键 词：多态性 单核苷酸 结核 肺 疾病易感性 

分 类 号：R135[医药卫生—劳动卫生]