利用基因枪法获得可遗传的抗除草剂转基因水稻植株  

Prodution of Herbicide resistant Transgenic Rice Plants from Immature Embryos Using Biolistic Method

作　　者：朱冰 黄大年[1,2] 杨炜[1,2] 薛锐 肖晗[1,2] 田文忠 李良才[1,2] 戴顺洪[1,2] 

机构地区：[1]中国水稻研究所 [2]中国科学院遗传研究所

出　　处：《中国农业科学》1996年第6期15-20,共6页Scientia Agricultura Sinica

基　　金：浙江省"八五"生物技术重点项目;美国洛克菲勒基金会资助项目

摘　　要：利用基因枪转化系统，将含有由ＣａＭＶ３５Ｓ启动子启动的ｂａｒ基因的转化质粒ｐＣＢ１导入水稻幼胚，经筛选、再生得到３株抗除草剂Ｂａｓｔａ的转基因植株。经ＰＣＲ及Ｓｏｕｔｈｅｒｎ分析，在转基因植株基因组中均检测到了ｂａｒ基因的整合；经ＲＮＡ点杂交分析，检测到了ｂａｒ基因在转基因植株中ＲＮＡ水平的表达。在转基因植株ＪＹ１１９－２的总计３２１株Ｔ１代自交后代中，有２７４株表现出除草剂抗性，４７株敏感。从该２７４株抗性植株中随机选取８株进行Ｓｏｕｔｈｅｒｎ分析，结果均检测到了ｂａｒ基因的整合。Plasmid pCB1, containing bar gene under the control of CaMV 35s promoter was introduced to rice immature embryo using biolistic method. Three basta resistant transgenic rice plants were regenerated after selection. The integration of the bar gene in the genome of the transgenic rice plants was shown by PCR and Southern analysis. RNA dot analysis demonstrated the mRNA expression of the bar gene at transcriptional level in transgenic plants. Among 321 T 1 progenies of transgenic plant JY119 2, 274 plants showed a high resistance to herbicide Basta, while the other 47 plants were sensitive. According to the Southern analysis of 8 randomly selected plants from those 274 Basta resistant plants, the bar gene has been stably inherited in T 1 progenies.

关 键 词：水稻 抗除草剂 基因枪 转基因植株 

分 类 号：S511.032[农业科学—作物学]