固相萃取-液相色谱法测定黄芪注射液2个黄烷类化合物的含量  

Determination of two flavanes in Huangqi injection using SPE coupled with RP-HPLC

作　　者：李宏 李建北[2] 张东明[2] 仲婕 

机构地区：[1]浙江九旭药业有限公司,金华321016 [2]中国协和医科大学中国医学科学院药物研究所,北京100050

出　　处：《药物分析杂志》2006年第8期1097-1099,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立黄芪注射液中2个黄烷类化合物的高效液相色谱含量测定方法。方法:采用 Agilent ZORBAX-SB-C_(18)分析柱(250mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸水溶液(43:57);流速:1mL·min^(-1);检测波长:277nm;最佳固相萃取洗脱条件减少了定量分析的杂质干扰。结果:9,10-二甲氧基紫檀烷-3-O-β-D-吡喃葡萄糖苷(HQ-1),线性范围为0.0888～1.4208μg,相关系数为0.9999;平均回收率为104.4%,RSD=0.4%。2′-羟基-3′,4′-二甲氧基异黄烷-7-O-β-D-吡喃葡萄糖苷(HQ-2),线性范围为0.0555～0.888μg,相关系数为0.9999;平均回收率为97.72%,RSD=1.4%。结论:该方法简便、准确、快速,结果可靠,为今后对黄芪注射液的质量控制研究提供了补充依据。Objective： To develope a method for the determination of 9,10 - dimethoxypterocarpan - 3 - O - β - D - glucopyranoside （ HQ - 1 ） and 2＇ - hydroxy - 3＇, 4＇ - dimethoxyisoflavan - 7 - O - β - D - gluco - pyranoside （ HQ - 2） in Huangqi injection using solid phase extraction coupled with RP - HPLC. Method： The Agilent ZORBAX SB -C18 column （250 mm × 4. 6 mm,5 μm） was used. The mobile phase was methanol-0. 1% phosphoric acid （43： 57） and the detection wavelength was 277 nm. The optimum conditions for extraction was studied in detail. The extraction time was shorter while the extraction efficiency was higher. The quantification could be done. Results：The method had good linear relationship within range 0. 0888 -1. 4208 μg,0. 0555 -0. 888 μg of HQ -1 and HQ -2 . The average recoveries were 104. 4% （ HQ - 1 ） and 97.72% （ HQ -2） respectively. Conclusion： The method is convenient, rapid, accurate and reliable, providing a basis for quality analysis of the Huangqi injection.

关 键 词：固相萃取 高效液相色谱 黄芪注射液 9 10-二甲氧基紫檀烷-3-O-β-D-吡喃葡萄糖苷(HQ-1) 2'-羟基- 3’ 4’-二甲氧基异黄烷-7—O-β—D-吡喃葡萄糖苷(HQ-2) 

分 类 号：R917[医药卫生—药物分析学]