固相萃取-高效液相色谱法测定血浆中强力霉素  

SPE-HPLC determination of doxycycline in plasma

作　　者：杨海峰[1] 万荣峰[1] 江善祥[1] 韩航如[2] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《药物分析杂志》2006年第8期1109-1111,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立血浆中强力霉素检测的固相萃取-高效液相色谱法(SPE-HPLC)。方法:血浆样品经 C_(18)固相萃取小柱提取后进样测定,色谱柱为 Kromasil C_(18)(250 mm×4.6 mm,5μm),柱温30℃,流动相为0.01mol·L^(-1)草酸溶液-乙腈-甲醇(55:25:20),流速为0,8 mL·min^(-1),检测波长为350 nm,进样量20μL。结果:血浆强力霉素浓度在0.1～5.0μg·mL^(-1)范围内线性关系良好(r=0.9996);最低检测限为0.1μg·mL^(-1);样品的平均回收率为93.9%～104%;日内和日间精密度均小于4.81%。结论:方法简便、准确可靠,适用于血浆强力霉素浓度测定及其药代动力学研究。Objective：To develop a solid phase extraction and high performance liquid chromatography （SPE - HPLC） method for the determination of doxycycline in pig plasma. Methods：Plasma samples were prepared with C18 solid phase extraction column. The chromatographic separation was performed on a Kromasil C18column（250 mm × 4. 6 mm,5 μm） at column temperature of 30 ℃. The mobile phase,a mixture of 0. 01 mol · L^-1 oxalic acid-acetonitrile- methanol（55： 25： 20）,was delivered at a flow rate of 0.8 mL · min^-1. The detection wavelength was 350 nm. Results： Excellent line relationship was obtained in the range of 0. 1 - 5.0 μg · mL^- 1 （ r = 0. 9996 ）. The determination limit of doxycycline was 0. 1 μg · mL^- 1. The average recovery rate was 93.9% - 104%. The intra - day and inter- day RSDs were less than 4.81%. Conclusion：The method is simple,accurate and can be used to determine the doxycycline concentration in plasma and for study of its pharmacokinetics.

关 键 词：固相萃取-高效液相色谱法(SPE—HPLC) 强力霉素 猪血浆 

分 类 号：R917[医药卫生—药物分析学]