HPLC法测定环磷腺苷针剂的含量及有关物质  

HPLC determination of the content of adenosinecyclophosphate and the related substances

作　　者：郭治安[1] 张小辉[1] 赵景婵[1] 

机构地区：[1]西北大学化学系,西安710069

出　　处：《药物分析杂志》2006年第8期1121-1123,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：陕西省教委基金项目(03JK075);陕西省自然科学基金(2005B18)

摘　　要：目的:采用高效液相色谱法测定环磷腺苷针剂的含量及有关物质。方法:采用 ODS C_(18)色谱柱(4.6 mm×150 mm,5μm),以甲醇-0.025 mol·L^(-1)磷酸二氢钠缓冲液(二水合磷酸二氢钠1.95g 加水至500 mL,用磷酸调 pH 至5.0)(10:90)为流动相,流速0.8 mL·min^(-1),于254nm 测定。结果:HPLC 法测定的线性范围为0.04～0.4 mg·mL^(-1),r=0.9999,最低检测限0.24 ng,对照品溶液在8 h 内稳定,日内和日间精密度(RSD<1.2%)良好。回收率为99.87%。结论:采用 HPLC 法测定环磷腺苷及有关物质的方法简便,结果可靠。Objective：To determine the content and the related substances of adenosine cyclophosphate by HPLC Methods： The column ： ODS C18 column （4.6 mm × 150 mm,5 μm） ; The mobile phase ： methanol - NaH2 PO4 buffer （0. 025 mol 2 L^-1monobasic sodium phosphate,adjust pH to 5.0 by phosphoric acid） （ 10： 90） ;The flow rate：0. 8 mL · min^- 1 ;The content of adenosine cyclophosphate and its related substances were detected at 254 nm on the same condition. Results ： The linear range of the determination by HPLC was 0. 04 - 0. 4 mg · mL ^- 1 ; The correlation coefficient was 0. 9999 ; The least detective range was 0. 24 ng; The sample solution was stable within 8 h ; The precision of intra - day and inter - day were good （ RSD 〈 1.2% ）. The recovery result was 99.87%. Conclusion ： The method to determine the content and related substances of adenosine cyclophosphate by HPLC is easy to operate and the results of the determination are accurate.

关 键 词：环磷腺苷 有关物质 含量 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]