EB荧光探针表征阿霉素及其转铁蛋白复合物与DNA的相互作用  

Investigation of the interaction between adriamycin/adriamycintransferrin and DNA with EB as fluorescence probe

作　　者：杨培慧[1] 苏章益[1] 陈晓翔[1] 

机构地区：[1]暨南大学化学系,广州510632

出　　处：《药物分析杂志》2006年第8期1124-1127,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：广东省自然科学基金(021190);广州市科技计划项目(200323-D2041)资助

摘　　要：目的：研究阿霉素（ADR）及其转铁蛋白复合物（ADR—Tf）与DNA作用机理的差异。方法：利用荧光光谱法，以溴化乙锭（EB）为荧光探针研究阿霉素及其转铁蛋白复合物与DNA的相互作用，采用Scatchard方程，对作用机理进行判别。结果：ADR、ADR—Tf与DNA的结合常数（K）分别为1．10×10^6，1．95×10^6；结合位点数（n）分别为4．3，2．6。ADR、ADR—Tf与DNA的作用方式有所不同，前者通过嵌插作用，而后者则为静电作用。结论：ADR—Tf并不具有ADR插入DNA的特征，表明了两者在抗癌机制上存在一定的差异。Objective：To investigate the difference of mechanism in which DNA interaction with adriamycin （ADR） and its compound with transferrin （ADR- Tf）. Method： Using ethidium bromide（EB） as fluorescence probe to study the interaction of ADR/ADR - Tf and DNA by fluorescence spectroscopic method,and estimate their interaction mechanism by Scatchard equation. Results-The binding constant K of ADR/ADR - Tf and DNA was 1.10 × 10^6 and 1.95 × 10^6 respectively ,and binding sites at 4. 3 and 2. 6 respectively. The mode of the interaction of ADR and DNA was not the same with that of ADR -Tf and DNA while the interaction of the former was in intercalation way but the latter was in static electrification way. Conclusion：ADR -Tf doesn＇t insert in the groove of DNA as ADR. It suggests that ADR and ADR - Tf have differences on their mechanism of anticancer.

关 键 词：阿霉素 转铁蛋白 DNA 溴化乙锭 荧光光谱法 相互作用 

分 类 号：R917[医药卫生—药物分析学]