重度烧伤患者免疫细胞凋亡及单核细胞HLA-DR抗原表达改变及意义  

Changes and significance of immunocyte apoptosis and the expression of HLA-DR on monocytes in severe burn patients

作　　者：李利根[1] 柴家科[1] 曹卫红[1] 李峰[1] 冯光[1] 

机构地区：[1]解放军总医院第一附属医院全军烧伤研究所,北京100037

出　　处：《西南国防医药》2006年第5期474-476,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的：探讨重度烧伤患者外周血免疫细胞凋亡率及单核细胞HLA—DR抗原表达的改变及意义。方法：16例大面积烧伤85％±11．02％TBSA患者，入院后48h行四肢削痂术，术前及术后1、7、14、28d，采用流式细胞术观察外周血淋巴细胞、中性粒细胞凋亡率及单核细胞HLA—DR抗原表达率的变化。结果：烧伤后淋巴细胞、中性粒细胞凋亡率即明显增加，术后1～7d进一步升高，随后逐渐降低；单核细胞HLA—DR表达率在烧伤后略有降低，术后持续处于较低水平。结论：重度烧伤及术后外周血免疫细胞凋亡率增加，但单核细胞HLA—DR的表达率降低，大面积烧伤患者进行必要的免疫功能监测和免疫调理治疗对烧伤的救治是有益的。Objective： To observe the changes and significance of immunocyte apoptosis and the expression of human leukocyte antigen （HLA- DR） on monocytes in severe burn patients. Methods： Sixteen severe burn patients（85 ± 11. 02）% TBSA were enrolled, the tangential excision was performed at 48 h after admission, the apoptosis changes of lymphocytes and neutrophils and the expression of HLA - DR on monocytes were detected with flow cytometry before and after operation at 1, 7, 14 and 28 days. Results： The apoptosis changes of lymphocytes and neutrophils were higher after burn and operation than that of normal group, the expression of HLA - DR on monocytes decreased after burn, while maintained lower levels after operation during the whole treatment. Coudusion：The immunocyte apoptosis increased and expression of HLA - DR is lower after severe burn and operation. Therefore, it is necessary to monitor and improve the immune function of patients with severe burn.

关 键 词：烧伤 免疫细胞 凋亡 

分 类 号：R644[医药卫生—外科学]