猕猴输精管内注射高分子聚合物HFMC后对睾丸组织结构的影响  

Histo-Pathological Effect on Testis Following Intra-Vas Deferens Injection of HFMC in Rhesus Monkey

作　　者：何亚平[1] 岳利民 罗自强[1] 张金虎[1] 姚先莹 李光蓉[1] 白寿昌[1] 邹淑茎 徐若璞 

机构地区：[1]华西医科大学基础医学院生理学教研室,中国科学院昆明动物研究所,中国科学院成都有机化学研究所

出　　处：《生物医学工程学杂志》1996年第3期193-196,共4页Journal of Biomedical Engineering

基　　金：四川省科委经费资助

摘　　要：本实验选用具有生育力成年雄性猕猴７只，在直视下行双侧ＨＦＭＣ输精管内注射，每侧剂量分别为３０ｍｇ１只，６０ｍｇ和１００ｍｇ各３只；于注射后２．５年和３．５年分别处死动物，取睾丸组织进行光镜和电镜观察．结果发现：猕猴注射ＨＦＭＣ２．５年后，睾丸光镜大部分曲细精管生精上皮结构完整，排列整齐。仅见局部少数管腔生精上皮层数减少，上皮细胞轻度水样变性等病理改变。电镜下曲细精管内除支持细胞内脂褐素增多，轻度基底膜增厚和精母细胞内质网扩张外，各级生精细胞，支持细胞及细胞间连接复合体等超微结构未见明显异常。注射ＨＦＭＣ３．５年后猕猴的光镜、电镜结果与注射后２．５年结果相似，但局部改变较２．５年组轻。上述结果表明：猕猴输精管内注射一定剂量ＨＦＭＣ节育不会引起睾丸组织的严重病理改变。但是，由于注射ＨＦＭＣ后，ＨＦＭＣ释放Ｈ＋及其对输精管的暂时阻塞，改变了精子生存的内环境，使睾丸出现局部轻度病理改变，随着ＨＦＭＣ逐渐溶解排出，睾丸功能相继恢复正常，配对产仔。为ＨＦＭＣ应用提供了安全性依据。This was a study of the histo-pathological effect on the testes of seven rhesus monkeys at the timeof and 3t years after the injection of high molecular polymer HFMC into their vas deferens on both sides. Itwas found light-microscopically that, years after HFMC injection, the spermatogenic epithelia of most testicular convoluted seminiferous tubules had various germ cells, but there were loci where the seminiferous tubules hadreduction of spermatogenic epithelia, separation and slight watery degeneration of germ cells in monkeys given ious dosages of HFMC. 3i years after HFMC inkction, similar histo-pathological changes were observed. Electron-microscopically,and years after the injection, no obvious abnormalities of germ cells, Sertoli cellsand intercellular junction in convoluted seminiferous tubules were found, except;ng an increase of lipofuscin in Sertoli cells, slight proliferation of basement membrane and ehdoplasmic reticulum enlargement in spermatocytes.The results suggest that the slight histo-pathological change may be due to the temporary blockage of vas deferens, the release of H+ from HFMC and hence the alteration in internal environment, but the function of testis can berestored following the dissolution and excretion of HFMC.

关 键 词：猕猴 HFMC 输精管 睾丸 生精上皮 避孕 

分 类 号：R169.41[医药卫生—公共卫生与预防医学] R318.08