用AFM观察小白鼠心肌核DNA片段的基因体外转录和垃圾DNA的相互作用  

AFM was Used to Observe Interaction between Mouse Cardiac Nuclear DNA Fragment in vitro Transcription and Junk DNA

作　　者：袁明秀[1] 张志宏[1] 李建伟[2] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]中国科学院化学研究所,北京100080

出　　处：《科技导报》2006年第9期46-50,共5页Science & Technology Review

摘　　要：用AFM直接观察、体外转录等实验技术组合,发现小白鼠(Balb/C)心肌体外转录状态的核DNA片段上的各种基因,处于垃圾DNA的特定的“转录平台”上。“转录平台”上的各种核活性基因的两端的调控序列,分别与特定开关蛋白质复合体结合(即可解离的开关蛋白质),中间的编码序列分别以非共价键特异结合可完全解离的转录活性因子等多种蛋白质;这些与核基因转录相关的蛋白质均由垃圾DNA的专一性蛋白质通路分别进行特异性正负反馈调控。Direct observation and in vitro transcription were used to study mouse （Balb/C） cardiac muscle genes which exist on transcription platform, Regulatory elements of two ends of active genes non-covalently bind proteins that were encoded by these genes, and these proteins bind small informative molecules to form dissolvable transcription factors （dissolvable switch proteins）. These transcription-related proteins are positively and negatively regulated by junk DNA-specific protein pathways.

关 键 词：垃圾DNA 专一性 蛋白质通路 正负反馈调控 转录平台 

分 类 号：Q75[生物学—分子生物学]