二硝基氟苯修饰的黑色素瘤细胞激活树突状细胞诱导特异性T细胞反应  

Dendritic Cells Activitized with 2,4-Dinitrophenyl-Modified Melanoma Cells Induced the Specific Immune Response

作　　者：杜楠[1] 李留树[1] 肖文华[1] 唐卓斌[1] 孙同柱[1] 

机构地区：[1]解放军总医院第一附属医院,北京100037

出　　处：《生物技术通讯》2006年第4期577-579,共3页Letters in Biotechnology

基　　金：国家自然科学基金项目(39900040)

摘　　要：目的:探索半抗原二硝基氟苯(DNP)修饰的恶性黑色素瘤细胞(恶黑)激活树突状细胞(DC)后,在体外诱导特异性T细胞反应的抗肿瘤效应。方法:采用DNP修饰恶黑细胞M3(H-2d),然后在体外激活BALB/c小鼠(H-2d)外周血来源的DC,用于激发自体的T细胞,观察对T细胞的增殖和特异性T细胞的杀伤功能。结果:经DNP修饰的M3细胞激活的DC,其诱发的T细胞增殖能力和对M3细胞的特异性杀伤效应均明显高于未修饰的M3细胞组和DC组。结论:DNP修饰M3所激活的DC可以诱导更强的恶黑特异性T细胞效应。Objective： To study the in vitro specific immune reponse induced by dendritic cells（DC） activitized with 2,4- dinitrophenyl（DNP）-modified melanoma cells. Methods： In vitro melanoma cells M3（H-2d） were modified with DNP, haptenized M3 melanoma cells were also able to activate DC collected from peripheral blood monocytes of BALB/c mice（H-2d） and cocuhured with autologous T cells. The proliferation of the T cells and their cytotoxic activity against DNP modified M3 melanoma cells were assessed and compared with those T cells induced by DC activated with M3 melanoma cells. Results： DC activated with DNP modified M3 were able to stimulate effectively T lymphocyte proliferation and the T cells could specially lysis M3 melanoma cells. Conclusion： DC activated with DNP modified melanoma cells can elicit activation of haptenized melanoma-specific T lymphocyte.

关 键 词：半抗原 二硝基氟苯 树突状细胞 T淋巴细胞 

分 类 号：R392[医药卫生—免疫学]