AB-8型树脂分离纯化葛根总黄酮研究  

Separation and Purification of Total Isoflavones Extracted from Pueraria Lobata Using AB-8 Type of Resin

作　　者：屈海宁[1] 蒋柏泉[1] 白兰莉[1] 

机构地区：[1]南昌大学环境科学与工程学院化工系,南昌江西330029

出　　处：《江西化工》2006年第3期104-106,共3页Jiangxi Chemical Industry

摘　　要：采用AB-8型大孔树脂对从葛根中提取的总黄酮产物进行分离纯化研究。考察了各种因素对树脂吸附和洗脱效果的影响。通过实验，得到了吸附的最佳工艺条件为：葛根黄酮上样液浓度6．6931mg／mL，上样液量为3倍树脂柱体积，PH值为5-6，吸附流速为1．0mL／mira；洗脱工艺条件为：洗脱液（70％乙醇）量为5倍树脂柱体积，流量为1．0mL／min。分离纯化后的葛根总黄酮产品纯度可达70％。The separation and purification of ca：tried out using AB - 8 type of resin in this orption of the resin were investigated. the total isoflavones extracted article. The effects of various Through from pueraria lobata were futher factors on the adsorption and des- the experiment, the optimal adsorption conditions were： the concentration of isoflavones, the volume, the pH value and the flow rate of the upstream solution were 6. 6931mg/mL, 3 times of volume of the resin bed, 5 - 6 and 1.0mL/min respectively and the optimal desorption conditions were： the volume and flow rate of the eluant （ 70wt% alcohol） were 5 times of the resin bed and 1.0mL/min respectively. The purity of the final isflavines product reached 70%.

关 键 词：葛根 总黄酮 树脂 吸附 纯化 

分 类 号：TQ28[化学工程—有机化工]