Aβ_(25-35)海马注射对大鼠神经细胞中NF-κB活性的影响  

Activation of NF-κB after β-amyloid injection into hippocampus of rats

作　　者：王秋艳 马涤辉[1] 陈微[1] 李晶[1] 

机构地区：[1]吉林大学第一医院神经内科,吉林省长春市130021

出　　处：《实用老年医学》2006年第5期341-343,共3页Practical Geriatrics

摘　　要：目的在体条件下观察β-淀粉样蛋白(β-amyloid,Aβ)对核因子κB(nuclearfactorκB,NF-κB)活性的影响,探讨二者之间的关系。方法以Aβ25-35及生理盐水做海马立体定向注射后,采用水迷宫、HE染色及免疫组织化学方法,观察实验动物学习记忆改变、脑组织病理形态变化及大鼠海马部位NF-κB活性的变化。结果Aβ组和生理盐水对照组通过水迷宫的潜伏期分别为(59·93±23·43)s和(8·56±4·81)s,差异显著(P<0·01),而Aβ组海马各区及齿状回可见大量的NF-κB阳性细胞,以齿状回和CA1区明显,与对照组相比,差异显著。结论在体条件下Aβ能激活NF-κB,提示NF-κB的活化与Aβ的神经细胞毒性有关。Objective To investigate the effects of β-amyloid（Aβ） on NF-κB in vivo. Methods One week after sterotaxis injection of Aβ25-35 and normal saline into CA1 subregion of hippocampus, the changes of their memory recovery, the hippocampus pathological abnormalities and the activity of NF-κB were evaluated with Morris test, hematoxylin-eosin staining and immunohistochemical assays. Results The latency of Aβ group（59. 93 ± 23.43 s） was significantly different from that of saline group（8. 56 ±4. 81 s）, while a large number of NF-κB positive cells were observed in hippocampus of Aβ group, especially in CA1 subregion and dentate gyrus, which were different from those of saline group. Conclusions Aβ could induce the activation of NF-κB, which is related to the neurotoxity of Aβ.

关 键 词：淀粉样Β蛋白 NF-ΚB 阿尔茨海默病 

分 类 号：R338.6[医药卫生—人体生理学]