离子交换色谱流动相组成对核糖核酸酶的折叠影响  

Effect of the composition of mobile phase on the refolding of reduced ribonuclease A by ion-exchange chromatography

作　　者：李敏[1] 段淑娥[1] 王彦[2] 耿信笃[2] 

机构地区：[1]西安文理学院化学系,陕西西安710065 [2]西北大学现代分离科学研究所,陕西西安710069

出　　处：《西安科技大学学报》2006年第3期372-375,共4页Journal of Xi’an University of Science and Technology

基　　金：西安文理学院专项科研基金资助项目(KY200330)

摘　　要：用离子交换色谱法(IEC)研究了还原变性核糖核酸酶(RNase A)折叠过程中,复性缓冲液种类及pH值、流动相中脲浓度及盐种类对还原变性核糖核酸酶复性的影响。发现pH为弱碱性,用PBS作为缓冲液能提高复性效率;当流动相中含有2.0 mol/L脲时复性产率较高;洗脱剂用NaC l时活性回收率较高。The refolding of the reduced RNase A was investigated by ion exchange chromatography （IEC）. The kinds of renaturation buffer and pH, Urea concentration in the mobile phase and the kinds of eluted salt was studied. It was found that the bioactivity yield in the phosphate buffer solution is better, and the peak obvious. 2.0 mol/L urea in the mobile phase was found to increase the bioactivity recovery. When the sodium chloride as eluted agent, the bioactivity recovery is the highest.

关 键 词：离子交换色谱 核糖核酸酶 折叠 脲 

分 类 号：O652.63[理学—分析化学]