对吸入麻醉药敏感性不同的果蝇脑神经元钾电流的研究  

Outward currents in larval neurons of three strains of Drosophila with different sevoflurane-sensitivity

作　　者：刘洋[1] 李彩群 廖大清[1] 刘进[1] 

机构地区：[1]四川大学华西医院麻醉与危重急救研究室,成都610041 [2]四川省乐山市马边县人民医院,614600

出　　处：《四川生理科学杂志》2006年第3期101-104,共4页Sichuan Journal of Physiological Sciences

基　　金：国家自然科学基金资助(No.30070732)

摘　　要：目的:以对吸入麻醉药具有不同敏感性的果蝇幼虫脑神经元为模型,利用膜片钳技术记录其钾电流,探讨吸入麻醉药的作用机制。方法:分离制备对吸入麻醉药敏感(S)、耐药(R)和野生(H)果蝇品系脑神经元,以膜片钳技术记录其全细胞钾电流。实验数据以“均数±标准差”表示,SPSS软件进行统计分析。结果:在果蝇晚三龄幼虫脑神经元上只记录到外向钾电流,未见其它电流成分。三品系脑神经元大小(细胞膜电容)没有显著性差异(P>0.05),而电流峰值有显著性差异:敏感>野生>耐药(P<0.05)。结论:钾通道可能是吸入麻醉药的中枢作用位点之一。Objective： Outward currents in larval neurons of the three strains of drosophila with high, normal and low sensitivity to sevofluranel were investigated. Methods： Isolated drosophila larval neurons were obtained from enzymatieally treated Drosophila larval brains. Patch clamp recording techniques were used to investigate the outward currents in Drosophila larval neurons. Results： In the whole-cell patch-damp mode, depolarizing pulses from 80 mV to ＋60 mV elicited outward currents. The outward currents recorded in the present investigation were sensitive to potassium channel blocker TEA, 4-AP and CsC1 proven to be potassium channel currents, I-V curves of outward currents in larval neurons of the three strains of Drosophila are also significantly different. Peak （Ipeak） current in the sevoflurane-sensitive group was much higher than those in the other groups （S： 2288. 6±317. 1 pA （n= 16）, H： 1538. 0±276. 6 pA （n=13）, R： 1111.1 a46. 2 pA （n =21）, respectively, P〈0. 05）. Membrane capacitances （Cm） of three strains of neurons were not significantly different （P〉0.05）. Conclusion： This study has shown the K＋ channels in sevoflurane-sensitive strain of fruitfly have high Ipeak. This indicates volatile anesthetics may act on K^＋ channels in the mechanisms of anesthesia. Key words： Drosophila melanogaster; Patch-clamp technique; K＋ currents; Svoflurane sensitivity

关 键 词：果蝇 膜片钳 钾通道电流 吸人麻醉药敏感性 

分 类 号：R614[医药卫生—麻醉学]