Two-photon excited spectroscopies of ex vivo human skin endogenous species irradiated by femtosecond laser pulses  

作　　者：陈建新 卓双木 罗天舒 赵敬军 

机构地区：[1]Key Laboratory of Optoelectronic Science and Technology for Medicine (Fujian Normal University),Ministry of Education,Fuzhou 350007 [2]Department of Skin,Affiliated Xiehe Hospital,Fujian Medical University, Fuzhou 350001

出　　处：《Chinese Optics Letters》2006年第10期598-600,共3页中国光学快报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China (No. 60508017)the Program for New Century Excellent Talents in Fujian Province Universitythe Natural Science Foundation of Fujian Province of China (No. A0510015)the Innovation Foundation for Young Technological Talents of Fujian Province (No. 2003J027)the Scientific Program of the Educational Department of Fujian Province (No. JA05215).

摘　　要：Two-photon excited spectroscopies from ex vivo human skin are investigated by using a femtosecond laser and a confocal microscope （Zeiss LSM 510 META）. In the dermis, collagen is responsible for second harmonic generation （SHG）; elastin, nicotinamide adenine dinucleotide （NADH）, melanin and porphyrin are the primary endogenous sources of two-photon excited autofluorescence. In the epidermis, keratin, NADH, melanin and porphyrins contribute to autofluorescence signals. The results also show that the SHG spectra have the ability to shift with the excitation wavelength and the autofluorescence spectra display a red shift of the spectral peaks when increasing the excitation wavelength. These results may have practical implications for diagnosis of skin diseases.Two-photon excited spectroscopies from ex vivo human skin are investigated by using a femtosecond laser and a confocal microscope （Zeiss LSM 510 META）. In the dermis, collagen is responsible for second harmonic generation （SHG）; elastin, nicotinamide adenine dinucleotide （NADH）, melanin and porphyrin are the primary endogenous sources of two-photon excited autofluorescence. In the epidermis, keratin, NADH, melanin and porphyrins contribute to autofluorescence signals. The results also show that the SHG spectra have the ability to shift with the excitation wavelength and the autofluorescence spectra display a red shift of the spectral peaks when increasing the excitation wavelength. These results may have practical implications for diagnosis of skin diseases.

关 键 词：Diagnosis Fluorescence PHOTONS Second harmonic generation Skin 

分 类 号：TN249[电子电信—物理电子学]