小片段红系特异增强子对MEL细胞中外源β珠蛋白基因表达调控的作用  

Small Erythroid Enhancer Fragment on the Expression of Transferred β-globin Gene in MEL Cells

作　　者：陶吉中[1] 刘德培[1] 韦启泽[1] 翟锦彬[1] 阎波 刘庆辉[1] 王晶[1] 贾佩臣[1] 梁植权[1] 

机构地区：[1]中国医学科学院基础医学研究所医学分子生物学国家重点实验室

出　　处：《高技术通讯》1996年第11期47-49,共3页Chinese High Technology Letters

摘　　要：在以往研究基础上，本文进一步采用了１００ｂｐ红系特异增强子，构建了反转录病毒重组体Ｎ＿２Ａ·β·Ｅ１００和Ｎ＿２Ａ·β·Ｅ１００，经Ψ－２和ＰＡ３１７两轮包装细胞系转染后，利用出芽的病毒粒子感染ＭＥＬ细胞，检测外源基因的整合与表达。结果表明１００ｂｐ和３６ｂｐ之间的差异序列对病毒滴度和前病毒整合无明显影响，但能使外源β珠蛋白基因的表达水平提高约一倍。Based on our previous work, we further used 100bp 5'HS2 erythroid enhancer in retroviral mediated globin gene transfer. We constructed the retrovirus vectors N2A.β. E100 and N2A·β·E100, which were packaged by -2 and PA317 packaging cell lines. Virus supernatants were used to infect MEL cells and RNase protection assay was performed. Results showed the different sequence between 100bp and 36bp had no significant influence on the virus titer and provirus integration, but could raise the expression level of transferred β-globin gene by about one fold.

关 键 词：β珠蛋白基因 红系特异增强子 细胞 

分 类 号：Q51[生物学—生物化学]