两个重组质粒在快生型大豆根瘤菌中的稳定性的比较  

Comparison of the Stability of Two Recombinant Plasmids in Rhizobium Fredii

作　　者：万钧[1] 覃筱婷[1] 周俊初 

机构地区：[1]北京农业大学生物学院微生物系,华中农业大学农业部农业微生物学重点实验室

出　　处：《高技术通讯》1996年第11期50-52,共3页Chinese High Technology Letters

基　　金：863计划

摘　　要：将两个复制子（ｒｅｐｌｉｃｏｎ）相同的重组质粒导入快生型大豆根瘤菌中，考察了得到的转移接合子在培养和共生条件下的稳定性。发现带有质粒ＲＫ２ｐａｒ基因的重组质粒ｐＴ２ＴＸ３Ｋ在快生型大豆根瘤菌中无论在培养还是共生条件下都可以稳定存在，而不带有该基因的重组质粒ｐＣＫ３在两种条件下都有一定程度的丢失。The stability of two recobinant plasmids in Rhizobi fredii was studied under cultured and symbiotic conditions. The results indicated that pT2TX3K was very stable under two conditions. pCK3 was unstable under two conditions. It suggested that pTR102 which is the vect of pT2TX3K should be a good tool ofgenetic engineering of rhizobia.

关 键 词：重组质粒 大豆 根瘤菌 稳定性 

分 类 号：S565.1[农业科学—作物学]