甲磺隆降解菌FLDA的分离鉴定及其降解特性研究  

ISOLATION AND IDENTIFICATION OF METSULFURON-METHYL DEGRADING STRAIN FLDA AND ITS DEGRADATING CHARACTERISTICS

作　　者：黄星[1] 何健[1] 潘继杰[1] 孙纪全[1] 顾立锋[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,南京210095

出　　处：《土壤学报》2006年第5期821-827,共7页Acta Pedologica Sinica

基　　金：国家自然科学基金"30500010"项目;江苏省自然科学基金"BE2003343;BE2002303"项目资助

摘　　要：从生产甲磺隆的农药厂内采取污泥,经驯化富集后筛选到一株能高效降解甲磺隆的细菌FLDA,根据表型特征、生理生化特性及16S rDNA序列同源性分析,将FLDA初步鉴定为假单胞菌(Pseudomonassp)。该菌能在含甲磺隆(30 mg L-1)的基础盐液体培养基中降解甲磺隆,5 d降解率达72.6%,该菌降解甲磺隆的最适pH为7.0,最适温度为30℃,该菌降解甲磺隆的速率和起始接种量呈正相关。酶的定域实验表明,该菌中甲磺隆水解酶为胞内酶。FLDA投加土壤,可提高土壤中甲磺隆的降解速率。A strain of FLDA capable of degrading metsulfuron-methyl aerobically was isolated from sludge collected from a pesticide（metsulfuron-methyl） plant. Based on analysis of its 16S rDNA, morphology, physiological and biochemical characteristics, strain FLDA was identified preliminarily as Pseudomonas sp. This strain of bactera could degrade 72.6% of 30mgL^-1 metsulfuron-methyl in liquid medium within 5days. The optimal pH and temperature for metsulfuron-methyl degradation was 7.0 and 30℃, respectively. The degradation rate was related positively to initial inoculation rate. Enzyme distribution experiment showed that the metsulfuron-methyl degradeing enzyme in the bacterium was endoenzyme. Strain FLDA could be used to degrade metsulfuron-methyl in soil.

关 键 词：甲磺隆 假单孢菌 生物降解 

分 类 号：S154.39[农业科学—土壤学]