石菖蒲有效成分配伍对Aβ损伤PC12细胞的保护作用  

Protective Effect of Combination of Effective Components in Acorus tatarinowii on PC12 Cell Injured by Beta Amyloid Protein

作　　者：江湧[1] 方永奇[1] 何玉萍[1] 

机构地区：[1]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2006年第5期335-338,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金(编号04300378);广东省中医药管理局基金(编号2040007)

摘　　要：目的观察石菖蒲有效成分β-细辛醚和丁香酚组合对β-淀粉样蛋白毒性损伤PC12细胞的保护作用,探讨中药有效成分配伍的优越性。方法观察给药后PC12细胞形态、细胞存活率、细胞内Ca2+浓度和NO的变化。结果β-细辛醚10μmol/L+丁香酚3μmol/L组合能有效减少细胞内Ca2+浓度,抑制NO的产生,提高细胞存活率。结论石菖蒲有效成分的配伍,能更好地发挥保护PC12细胞免受β淀粉样蛋白毒性损伤的作用。Objective To observe the effect of β - asarone combined with eugenol against PC12 cell injury induced by amyloid beta protein （Aβ25-35） and to explore the advantage of compatibility of monomers in herbs. Methods PC 12 cells injury were induced by Aβ25 - 35. The morphological features and survival rate of PC 12 cells as well as the changes of intracellular Ca^2＋ concentration and NO concentration were observed after administration. Results The optimum combination was 10 μmol/L [3 - asarone ＋ 3 μmol/L eugenol, which can decrease the concentration of intracellular Ca^2＋ , inhibit the production of NO and increase the survival rate of PC12 cells. Conclusion The combination of effective components in Acorus tatarinowii has a better protective effect on Aβ25-35 - induced PC12 cell injury than Acorus tatarinowii.

关 键 词：石菖蒲 Β-淀粉样蛋白 Β-细辛醚 丁香酚 PC12细胞 

分 类 号：R285.5[医药卫生—中药学]