共振瑞利散射法测定孔雀石绿  

Determination of trace malachite green by resonance rayleigh-scattering method

作　　者：范翔[1] 吕昌银[1] 刘运美[2] 何爱桃[1] 贺元文[1] 宁玲[1] 

机构地区：[1]南华大学公共卫生学院卫生化学检验学教研室,湖南衡阳421001 [2]南华大学生命科学与技术学院,湖南衡阳421001

出　　处：《中国卫生检验杂志》2006年第10期1158-1159,1169,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立共振瑞利散射测定痕量孔雀石绿的新方法。方法：在硫酸介质中，孔雀石绿与磷钼酸根阴离子形成离子缔合物而产生共振瑞利散射，据此建立了以磷钼酸根阴离子为载体，共振瑞利散射测定痕量孔雀石绿的新方法。结果：方法的线性范围为0．18-5．0μg／ml，检出限为55ng／ml。将其用于渔池水和水产品中痕量孔雀石绿加标测定，回收率分别为88．7％-106．5％和87．4％-95．5％。结论：方法灵敏度高，分析速度快，仪器设备简单，分析成本低，检测渔池水和水产品中孔雀石绿，结果满意。Objective：To develop a novel method for the determination of trace malachite green （MG） in samples. Methods： In the H2 SO4 medium, MG reacted with phosphomolybdic acid （PMA） to form an ion - associate. The resonance rayleigh scattering （RRS） intensities of the ion -associate enhanced with the MG increasing. Based on those, RRS was proposed for the determination of trace MG. Results：The intensity of RRS was directly proportional to the mass concentration of MG in the range of 0. 18 - 5. 0 μg/ml, and the detection limit was 55 ng/ml. The recovery rate in fish and water was 87. 4% - 95. 5% and 88.7% - 106. 5%, respectively. Conclusion ：The method is sensitive, selective and rapid with simple instrument. Satisfactory result is obtained for the determination of trace MG in sample.

关 键 词：孔雀石绿 共振瑞利散射 磷钼酸 渔用药物 

分 类 号：O657.3[理学—分析化学]