与人遗传病相关(GCCT)_n·(CAGG)_n重复序列的分子克隆  

Molecular cloning on(GCCT)_n·(CAGC)_n repeat sequences associated with human genetic diseases

作　　者：蔡禄[1] 王晶妍[1] 赵秀娟[1] 

机构地区：[1]内蒙古科技大学生物与化学工程学院,内蒙古包头014010

出　　处：《包头钢铁学院学报》2006年第2期194-196,共3页Journal of Baotou University of Iron and Steel Technology

基　　金：国家自然科学基金资助项目(30460038);内蒙古自然科学基金重点资助项目(200508010102);内蒙古高等学校科学基金资助项目(NJ02116).

摘　　要：克隆与人遗传病相关的(GCCT)n.(CAGG)n重复序列.通过化学合成重复序列及两侧接头序列,两侧接头有EcoRⅠ,BamHⅠ和PleⅠ酶切位点,EcoRⅠ,BamHⅠ位点用于将重复序列插入pUC18制成的载体,PleⅠ酶切位点可方便地将重复序列从重组质粒中取出.为进一步研究和人遗传病相关的重复序列的结构特性及该类遗传疾病的分子机理奠定基础.（GC CT） n · （CAGC）n repeat sequences associated with human genetic diseases were cloned. There exist EcoR Ⅰ, BamH Ⅰand Pie Ⅰsites in chemically synthesized repeat sequences and adaptors .The sites of EcoR Ⅰand BamH Ⅰ were used to insert repeats sequences into vector made from pUC18, while Pie Ⅰ sites can be used to take out repeat sequences from recombined plasmid easily. This work was the basis of the further investigation on structural feature of repeat sequences and molecular mechanism of this kind of genetic diseases.

关 键 词：GCCT 重复序列 分子克隆 人遗传病 

分 类 号：Q61[生物学—生物物理学]