6α-甲基可的松C_(1,2)微生物脱氢反应的研究  

Study on Reaction Conditions for C_(1,2) Dehydrogenation of 6α-Methylcortisone by Arthrobacter Simplex

作　　者：葛翔[1] 龚万本[1] 王海清[1] 关怡新[1] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027

出　　处：《化学反应工程与工艺》2006年第4期344-349,共6页Chemical Reaction Engineering and Technology

基　　金：浙江省科技计划(2005C31028);杭州市科技计划项目(200433124)

摘　　要：利用简单节杆菌对6α-甲基可的松C1,2位脱氢，考察了脱氢反应温度、pH值以及底物的投料浓度和方式对底物转化率的影响，得到了较好的反应条件为脱氢反应温度33℃，pH值7．0～7．2，拟结晶投料方式增加底物的投料浓度。在3．7L发酵罐中进行脱氢反应初步放大试验，得到了菌体生长及底物转化率曲线。利用遗传算法对培养基组成进行了优化，在摇瓶培养中当投料浓度为1．0％时，底物的转化率提高到94．51％。Arthrobacter simplex was applied in the C1.2 dehydrogenation of 6α-methylcortisone to produce 6α-methylprednisolone. Experimental parameters of this biotransformation including dehydrogenation temperature, initial substrate pH and concentration of 6α-methylcortisone were investigated in detail and optimum reaction conditions （dehydrogenation temperature 33 ℃, initial substrate pH 7.0-7.2, 6α-methylcortisone concentration 1.0%） were obtained. Furthermore, the scale up of dehydrogenation was carried out in a 3. 7 L fermentor and the course of arthrobacter simplex growth and 6a-methylcortisone conversion were obtained. Finally, the optimization method of genetic algorithm was used to optimize ingredient of substrate, and 94.51% of 6α-methylcortisone conversion was obtained under the optimized conditions when the initial 6α-methylcortisone concentration was 1.0% in shaking flasks.

关 键 词：6α-甲基可的松 脱氢反应 简单节杆菌 

分 类 号：TQ467.8[化学工程—制药化工]