IL-1α对体外培养人牙囊细胞TNF-α表达的影响  

Regulation of secretion of TNF-α in human dental follicle cells by IL-1α.

作　　者：毕迎春[1] 林珠[1] 金作林[1] 钱红[1] 陈学鹏[1] 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032

出　　处：《临床口腔医学杂志》2006年第10期579-581,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助项目(30400510)

摘　　要：目的:研究白细胞介素-1(αinterleukin-1α,IL-1α)对体外培养的人牙囊细胞(dentalfolliclecells,DFC)分泌肿瘤坏死因子-(αtumornecrosisfactor-α,TNF-α)的影响,探讨TNF-α在牙齿萌出过程中的作用。方法:原代培养人牙囊细胞;取3～6代细胞,培养基中加入IL-1α,在不同浓度和时间点,MTT检测细胞增殖,免疫组化法检测细胞中TNF-α的表达,SandwichELISA测定培养上清中TNF-α的含量。结果:IL-1α在25ng/ml,促进人牙囊细胞的增殖,IL-1α与牙囊细胞共孵育,可使TNF-α表达和分泌增强。结论:IL-1α有增强人牙囊细胞TNF-α表达的作用。Objective：To study the effects of interleukin-1α （IL-1α） on the secretion of tumor necrosis factor-α （TNF-α） in human dental follicle cells. Method： The culture of human dental follicle cells was established, and the third to sixth passage cells were incubation with IL-1α for both time and dose dependent studies. MTT assays were used to evaluate the cells proliferation ; enzyme linked immunosorbent assays were used to measure the amount of TNF-α in the conditioned mediums and immunohistochemical staining technique was used to observe the expression of TNF-α. Result： This study showed that proliferation activity and the secretion of TNF-α increased significantly after incubation of the human dental follicle cells with 25 ng/ml IL-1α. Conclusion： IL-1α can increase the expression of TNF-α of human dental follicle cells at some stage during tooth eruption.

关 键 词：牙囊细胞 白细胞介素-1Α 肿瘤坏死因子-α 牙齿萌出 

分 类 号：Q813.1[生物学—生物工程]