17β-雌二醇调控骨外膜来源成骨样细胞雌激素受体及相关受体α基因表达  

Effect of 17β-estradiol on the gene expression of estrogen receptors and estrogen related-receptor α in the periosteum-derived osteogenic cells

作　　者：黄曼婷[1] 刘勇[2] 林守清[1] 邱贵兴[2] 

机构地区：[1]北京协和医院妇产科,100730 [2]北京协和医院骨科,100730

出　　处：《中华骨科杂志》2006年第9期618-621,共4页Chinese Journal of Orthopaedics

基　　金：863项目"人类重要生理活性及具有药物开发前景的功能基因研究"的子课题:骨质疏松相关功能基因的大规模筛选和鉴定(2002BA711A01-07)

摘　　要：目的探讨雌激素受体(estrogenreceptors,ER)及雌激素相关受体α(estrogenrelated-re-ceptorα,ERRα)基因在骨外膜来源成骨样细胞的表达及其与雌激素调控的关系。方法6周龄、雌性Wistar大鼠颅骨骨外膜来源的成骨样细胞分别在含有0.01、0.05、0.1、0.25、0.5、0.75、1、5、10nmol/L9种浓度17β-雌二醇(17β-E2)或对照的成骨诱导培养液中培养7d,提取细胞总RNA,采用RT-PCR法分析ER及ERRα基因的表达情况。结果ERα基因表达阴性,ERβ基因呈弱表达。从0～0.25nmol/L范围内可以看到ERβ的表达水平有上升趋势。5nmol/L和10nmol/L雌激素组ERβ的表达水平显著低于其他雌激素组(P<0.01)。ERRα表达阳性,各雌激素组的表达水平均显著高于对照组(P<0.01)。0.25nmol/L组的ERRα表达水平最高、0.5nmol/L组次之,两组的表达水平显著高于其他雌激素组(P<0.05);10nmol/L组表达水平显著低于其他雌激素组(P<0.05)。结论雌激素上调了骨外膜来源成骨样细胞ERRα的表达水平,低水平的雌激素有可能上调ERβ的表达水平。血清17β-E2水平维持在早、晚卵泡期的生理浓度有可能获得最佳的骨保护疗效。Objective To study the gene expression of estrogen reeeptors（ER） and estrogen relatedreceptor α （ERRα） in the periosteum-derived osteogenic cells and its relationship with the regulation of es- trogen. Methods The 6-week-old female Wistar rat ealvaria periosteum-derived osteogenic cells were divided 10 groups and cultured in different medium for 7 days. One group severved as control group, and cultured in osteogenic inducing medium. The other 9 group severved as experimental group, and the culture medium contained 0.01, 0.05, 0.1, 0.25, 0.5, 0.75, 1, 5, 10 nmol/L 17β-estradiol（17β-E2）. All RNA was ex- tracted, and then using RT-PCR assay to determine the effects of 17β-E2 on the mRNA expression level of ER and ERRcc Results ERα expression was negative. ERβ expression level was relatively low. There was gradually increasing trend for the expression level of ERβ from 0 to 0.25 nmol/L 17β-E2 treatment. The ERβ expression level of 5 and 10 nmol/L groups was significantly lower than the other 17β-E2 treatment groups. ERRα expression was positive. The expression level of 17β-E2 treatment group was significantly higher than control group （P〈 0.01 ）. 0.25 nmol/L group has the highest expression level, followed by 0.5 nmol/L group. Both of them have significantly higher expression level than the other 17β-E2 treatment groups. The expression level in 10 nmol/L group significantly lower than that in the other 17β-E2 treatment groups. Conclusion Estrogen may increase the ERRα expression level of periosteum-derived osteogenic cells. Low concentration of estrogen may increase the ERβ expression level. Maintaining serum 17β-E2 concentration at physiological level of the early and late follicular phase may obtain the optimal bone-protecting effect.

关 键 词：雌二醇 受体 雌二醇 骨膜 成骨细胞 

分 类 号：R580[医药卫生—内分泌]