CⅡ变构肽对胶原性关节炎T细胞免疫的影响  

Impact of altered CⅡpeptide on T cell response in collagen-induced arthritis

作　　者：姚中强[1] 李萍[2] 栗占国[1] 

机构地区：[1]北京大学人民医院风湿科,100044 [2]吉林大学中日联谊医院风湿科

出　　处：《中华微生物学和免疫学杂志》2006年第9期846-849,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金面上项目(30271223);重点项目(30430290)部分资助

摘　　要：目的探索连续替换CⅡ多肽中268-270位氨基酸的变构肽对胶原性关节炎(CIA)抑制作用的可能机制。方法CⅡ268-270变构肽90μg静脉注射,每周1次治疗CIA大鼠;设置无关肽对照组和空白对照组。ELISA方法检测大鼠血清TH1／TH2细胞因子水平及抗CⅡ抗体及其亚型。荧光实时定量RT-PCR检测调节性T细胞标记Foxp3 mRNA表达。结果CⅡ268-270变构肽、无关肽及空白对照组血清IFN-γ水平分别为41．42±7．32、54．63±11．15和57．86±5．64 pg/ml,变构肽可降低大鼠血清IFN-γ水平(P<0．01)。变构肽、无关肽及空白对照组血清IL-10水平分别为40．18±5．13、31．09±5．04和30．19±5．02 pg/ml,变构肽可以提高大鼠血清IL-10水平(P<0．01)。变构肽、无关肽及空白对照组抗CⅡIgG2a抗体滴度分别为0．5±0．21、1．1±0．27和1．3±0．29,变构肽可以显著降低抗CⅡIgG2a抗体滴度(P<0．01)。变构肽、无关肽及空白对照组抗CⅡIgG1抗体滴度分别为1．2±0．24、0．58±0．11和0．6±0．12,变构肽可以显著上调抗CⅡIgG1抗体滴度(P<0．01)。变构肽、无关肽及空白对照组Foxp3 mBNA表达丰度分别为33．62±12．12、10．05±6．08和9．73±6．03,变构肽可以显著上调大鼠Foxp3 mRNA表达丰度(P<0．01)。结论CⅡ268-270变构肽可以调节CIA中异常的TH1/TH2细胞因子平衡,可以抑制IgG2a型抗CⅡ抗体产生,诱导Foxp3阳性T细胞产生。Objective To explore the mechanism of the inhibitory effect of CⅡ268-270 altered peptide on collagen-induced arthritis. To study its effect on TH1/TH2 balance, anti-CⅡ IgG antibody isotypes and regulatory T celia. Methods The altered peptide was given intravenously with a dose of 90μg once a week. Peptide control and blank control were treated with similar approach. The serum TH1/TH2 cytokines were measured by ELISA. The anti-CⅡ antibody and its isotypes were measured with ELISA. The induction of regulatory T cells were assayed by real-time RT-PCR analysis of its marker Foxp3. Results The serum IFN-γ in rats treated with altered peptide, control peptide and blank control were 41.42±7.32, 54.63±11.15 and 57.86±5.64 pg/ml respectively （P 〈 0.01）. The serum IL-10 in rats treated with altered peptide, control peptide and blank control were 40.18±5.13, 31.09±5.04 and 30.19±5.02 pg/ml respectively （P 〈 0.01）. The Foxp3 mRNA abundance in spleen mononuclear cells of rats treated with altered peptide, control peptide and blank control were 33.62±12.12, 10.05±6.08 and 9.73±6.03 respectively （P 〈 0.01）. Conclusion CⅡ268-270 altered peptide could regulate the balance of TH1/TH2 cytokines. It could decrease anti-CⅡ IgG2a antibody. It could also up-regulate Foxp3^＋ T cells in vivo.

关 键 词：胶原性关节炎 变构肽 调节性T细胞 辅助性T细胞 

分 类 号：R593.22[医药卫生—内科学]