T淋巴细胞介导急性心肌梗死后心肌IL-6的表达  

T lymphocytes mediated expression of interleukine-6 after acute myocardial infarction

作　　者：张锦[1] 廖玉华[1] 程翔[1] 陈静[1] 陈鹏[1] 高翔[1] 郭张强[1] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所,武汉430022

出　　处：《临床心血管病杂志》2006年第10期621-623,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:30370574)

摘　　要：目的:探讨心肌梗死后介导自身免疫应答和细胞因子分泌的细胞类型。方法:体外分离同源大鼠T细胞和树突状细胞,与大鼠心肌肌球蛋白共培养,使T细胞活化,然后将活化的、未活化的T细胞分别过继转输给同源大鼠(转输组、对照组),观察3d、1周和4周后心肌的病理改变,分别采用免疫组织化学和RT-PCR的方法检测各时间点白细胞介素-6(IL-6)蛋白和mRNA的表达。结果:转输组大鼠心肌有不同程度地炎性细胞浸润,主要为淋巴细胞,偶见心肌水肿、变性及坏死,炎性细胞浸润以1周最明显,呈弥漫性;对照组大鼠心肌未见类似病理改变。转输组IL-6mRNA和蛋白表达在转输后3d开始升高,1周末达到高峰,4周末开始下降,1周末和4周末心肌组织的IL-6mRNA和蛋白表达均明显高于对照组(P<0·01)。结论:T细胞介导心肌自身免疫性炎症的发生,同时也促使心肌细胞致炎因子IL-6的表达,共同导致急性心肌梗死后心肌细胞的损伤。Objective：To investigate the type of immunological cells which mediate the autoimmune response and secrete cytokines after acute myocardial infarction （AMI）. Method：T lymphocytes and dendritic cells were respectively purified from spleens of Lewis rats. After coculturing in the absence or presence of cardiac myosin （20 mg/L）, sensitized or unsensitized T lymphocytes were transferred into naive syngeneic Lewis rats by a single tail vein infusion, respectively. Recipient rats were killed at 3 day, 1 and 4 weekend after activated T cells transfusion to perform histopathological studies. In addition, mRNA and protein levels of interleukin-6 （IL-6） in myocardial tissues were determined by RT-PCR and immunohistochemistry respectively. Result： T lymphocytes infiltration and occasional myocardium necrosis were observed in the rats after transferred activated T cells, the most markedly at the 1st weekend and not detected in the rats received unactivated T cells. IL-6 were detected at 3 days, the mRNA and protein expression were elevated markedly at 1 week and decreased at 4 weeks. Conclusion：T lymphocytes mediate the inflammatory response and expression of IL-6 after AMI.

关 键 词：心肌梗死 T淋巴细胞 白细胞介素-6 

分 类 号：R542.2[医药卫生—心血管疾病]