流动腔底面上内皮细胞图型化方法的初步研究  

Micropatterning of endothelial cells on poly-dimethylsiloxane flow channel bottom

作　　者：张莉[1] 韩亚刚[1] 蒋稼欢[1] 蔡绍皙[1] 

机构地区：[1]重庆大学生物工程学院生物力学与组织工程教育部重点实验室

出　　处：《第三军医大学学报》2006年第19期1927-1930,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(10272122)~~

摘　　要：目的体外模拟动脉粥样硬化好发部位复杂的血液流动状态,在流动腔底部形成微图型化的细胞,以获取单个细胞或确定点上细胞群的信息。方法利用软刻法制作带后向台阶的PDMS流动腔和PDMS印章,再通过微接触压印在流动腔底部形成图型化的纤粘蛋白结构,细胞选择性生长在有蛋白的表面上。结果获得了1个光学性能良好并能模拟体内特殊流形的流动腔及各种尺寸大小的印章,在腔底形成了纤粘蛋白图型和细胞小岛。结论该加工方法快速、简便,制作的PDMS流动腔可进一步用于体外细胞力学行为的实时研究。Objective To simulate the blood flow features of the susceptible sites of atherosclerosis in vitro. The cell micro-patterning is to be obtained on the bottom of channel for the information about single cell or cell groups. Methods Poly-dimethylsiloxane （PDMS） flow channel and PDMS stamp were fabricated by soft lithography. Fibronectin pattern was formed on chamber bottom by micro-contact printing method and cells selectively attached on fibronectin pattern. Results The PDMS-patterned chamber that possessed favorable optical property and the PDMS stamps with different sizes were obtained. Cells attached on fibronectin pattern presented square array. Conclusion The fabricated methods are quick and simple. The chamber could be used for the advanced realime investigation of cell mechanical behavior in vitro.

关 键 词：流动腔 聚二甲基硅氧烷(PDMS) 图型化 微接触压印(μCP) 数字模拟 

分 类 号：R318.01[医药卫生—生物医学工程] R322.121[医药卫生—基础医学]